Oxford, October 2023
We are looking for an individual to join our team as Production Laboratory Technician and cover all aspects of producing high quality products sold to biopharmaceutical companies and research organisations around the globe.
The detailed job description can be found using this link.
Apply by sending your CV to firstname.lastname@example.org.
Oxford, September/October 2023
Monosaccharide analysis is a regulatory requirement laid out in the ICH Q6B guidelines for the characterisation of biopharmaceuticals. This information can be used at all stages of drug development as a method of determining the type of glycosylation (N-linked and/or O-linked) and the extent to which glycosylation has occurred. It can also be used to demonstrate consistency between batches for QC lot release during the manufacturing process.
Follow the workflow below for monosaccharide quantitation.
Archana Shubhakar, Head of Business Development at Ludger, will be attending the 15th Annual PEGS Europe in Lisbon, Portugal from November 14th to 16th.
Archana will be presenting a poster describing a comprehensive strategy for the analysis of glycosylation, designed to satisfy the regulatory requirements outlined by EMA, FDA and ICH Q6B guidelines and ensure the potency and clinical safety of the biopharmaceutical.
Please contact us if you would like to meet Archana at the event
Glycosphingolipids (GSLs) are the most abundant and diverse class of glycolipids in animals (and are also present in fungi, plants, and invertebrates). Glycans present in GSLs have important roles in physiology and pathology. The ability to identify and measure GSLs is important for research in developmental neurobiology as well as lysosomal storage diseases such as Tay-Sachs and Gaucher’s disease. There is also growing interest in GSLs as possible targets for immunotherapy.
Ceramide glycanase is an enzyme used to release glycans from GSLs to enable their characterisation. It cleaves glycans including GM1, GM2, and GM3 by cleaving the β-glycosyl linkage. Glycans can then be labelled using LudgerTag labelling technology. We have purified ceramide glycanase from Hirudo medicinalis and offer this in a kit along with buffer and GM1 glycolipid substrate.
Follow the workflow below for the analysis of GSLs.
Purified labelled samples can be analysed using LudgerSep Amide HILIC HPLC column (LS-N2-4.6x150).
All the products mentioned in it are part of our catalogue and all their corresponding technical information can be found on our website. If you require any further information, please contact us at email@example.com.
Oxford, July/August 2023
Ludger V-Tag system is designed for the analysis of glycopeptides generated from the digestion of therapeutic glycoproteins such as monoclonal antibodies (mAb). It is comprised of two steps which can be completed in 2 hours (see workflow below). The first step involves the labeling of peptides and glycopeptides in a protease digest using a novel fluorophore that has been synthesized from 2-amino-1-naphthalenesulfonic acid. The second step is the enrichment and clean-up of the labeled glycopeptides using a hydrophilic interaction liquid chromatography (HILIC) cartridge.
- Integrates with Peptide Mapping Workflow without requiring extra steps for glycan release.
- Minimal Sample Amount - As little as 10 μg of glycoprotein (IgG) is required.
- Glycopeptides are enriched without degradation to preserve the glycosylation patterns and structures.
- Quick turn-around - labeling and glycopeptide enrichment is completed in 2 hours.
- Orthogonal Analysis - V-Tag labeled glycopeptides can be analysed by MALDI-MS and (U)HPLC to give you reliable glycan identification and quantitation.
- Validated for GMP Labs - Validated to ICH Q2(R1) standards and tested in GMP level glycoprofiling labs.
- Reliable mAb Glycoprofiling - Provides data comparable to gold-standard glycoprofiling methods based on 2-AB or 2-AA labeling.
- Automatable for High-Throughput Studies - The procedure is scalable and compatible with 96-well plate-based assays, enabling high-throughput studies using a liquid-handling robot.
We are pleased to announce the launch of a new size LudgerTag DMB Sialic Acid Release & Labeling Kit (LT-KDMB-96)
This kit has been developed for the quantitative analysis of sialic acids and contains all the reagents necessary for the release of sialic acids from glycoproteins and their conjugation with DMB dye by an amination-cyclization reaction. Its larger kit size is ideal for high-throughput automated workflows used for the analysis of biological samples such as blood serum or plasma (see our workflow below).