Ludger DMB Sialic Acid Analysis

DMB sialic acid release and labeling kit

Use the LudgerTag DMB kit (LT-KDMB-A1) to release and label sialic acids from your samples and compare to standards (N-acetyl neuraminic acid (Neu5Ac), N-glycolyl neuraminic
acid (Neu5Gc), 5-N-acetyl-9-O-acetyl neuraminic acid (Neu5,9Ac2) and a sialic acid reference panel) by (U)HPLC analysis.

- Release of sialic acids from glycoproteins or pre-released glycans and subsequent labeling with 1,2-diamino-4,5-methylenedioxybenzene (DMB).
- This kit can be used for routine sialic acid analysis and quantitation of Neu5Ac and Neu5Gc.
- Kit sufficient for 22 analyses.


Ludger Sialic Acid Analysis

  Product/Ordering Information:

Release and label sialic acids with DMB:

  • LudgerTag DMB sialic acid release and labeling kit

Sialylated Positive Controls:

  • Fetuin Glycoprotein Standard
  • LudgerBioQuant GPEP A2G2S2 glycopeptide standard
  • 5-N -acetyl-9-O-acetyl neuraminic acid standard (Neu5,9Ac2)
  • N -acetylneuraminic acid (Neu5Ac or NANA) quantitative standard
  • N -glycolylneuraminic acid (Neu5Gc or NGNA) quantitative standard
  • Sialic acid reference panel

HPLC Analysis:

  • LudgerSep R1 HPLC Column

UHPLC Analysis:

  • LudgerSep uR2 UHPLC Column


WCBP Sialic Acid Poster

Analysis of Sialic Acids in Biopharmaceuticals

Hendel J, Royle L, Kozak RP, Fernandes DL

Presented at:
WCBP 2017: 21st Symposium on the Interface of Regulatory and Analytical Sciences for Biotechnology Health Products
Washington D.C., United States. January 24-26th 2017


Technical Advice

Dr. Jenifer Hendel

Dr. Jenifer Hendel
Senior Scientist

Quotations and Orders

Ludger DMB Sialic Acid Release and Glycan Labeling Kit

Sales Team