Separation

Separation

LudgerSep™ range offers a variety of HPLC and UHPLC columns and buffers to support reliable, quantitative, and robust analysis of released glycans, sialic acid and monosaccharide analysis


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LudgerSep C buffer x4 concentrate

LudgerSep C buffer x4 concentrate

LS-C-BUFFX4

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50mL of x4 LS-C buffer concentrate (2.0M ammonium formate buffer, pH 9.0). This can be diluted with water and acetonitrile to prepare LS-C solvent (500 mM ammonium formate buffer pH 9, 20% acetonitrile v/v) used in WAX (weak anion exchange) HPLC analysis of LudgerTag fluorophore and UV-chromophore labeled glycans.

LudgerSep C2 anion exchange HPLC Column

LudgerSep C2 anion exchange HPLC Column

LS-C2-4.6x150

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LudgerSep™C2 HPLC columns contain particles with a macroporous polymeric anion exchange coating optimized for anion exchange chromatography of complex glycan mixtures. The LudgerSep strong anion exchange column is used for determining the charge profile of 2-AB and 2-AA labeled glycans as part of biopharmaceutical QC procedures.

LudgerSep N1 amide Guard Column

LudgerSep N1 amide Guard Column

LS-N1-4.6x10

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Analysis and purification by HPLC of LudgerTag™ fluorophore and UV-chromophore labeled glycans. LudgerSep™ N1 HPLC columns contain particles with a polymeric amide coating optimized for high resolution chromatography of complex glycan mixtures.

LudgerSep R BPT solvent x10 concentrate

LudgerSep R BPT solvent x10 concentrate

LS-R-BPTX10

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50 mL of x10 LS-BPT (butylamine/orthophosphoric acid/tetrahydrofuran) solvent.

LudgerSep R1 HPLC column

LudgerSep R1 HPLC column

LS-R1-4.6x150

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Analysis by HPLC of sialic acid variants fluorophore labeled with DMB (1,2-diamino-4,5-methylenedioxybenzene.2HCl) using the LudgerTag DMB Sialic Acid Labeling Kit.

LudgerSep uR2 UHPLC column - monosaccharide

LudgerSep uR2 UHPLC column - monosaccharide

LS-UR2-2.1x50

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Analysis of monosaccharides labeled with 2-aminobenzoic acid (2-AA) using UHPLC.

LudgerSep C2 anion exchange HPLC Column

LudgerSep C2 anion exchange HPLC Column

LS-C2-4.6x50

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A strong anion exchange column used for determining the charge profile of 2-AB and 2-AA labeled glycans as part of biopharmaceutical QC.

LudgerSep N buffer x40 concentrate

LudgerSep N buffer x40 concentrate

LS-N-BUFFX40

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50ml of x40 LS-N buffer concentrate (2.0M ammonium formate buffer, pH 4.4). This can be diluted with water to prepare LS-N buffer (50 mM ammonium formate buffer, pH 4.4) used in amide or HILIC (hydrophilic interaction liquid chromatography) HPLC analysis of LudgerTag fluorophore and UV-chromophore labeled glycans.

LudgerSep N1 amide HPLC Column

LudgerSep N1 amide HPLC Column

LS-N1-4.6x250

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Analysis and purification by HPLC of LudgerTag™ fluorophore and UV-chromophore labeled glycans. LudgerSep™ N1 HPLC columns contain particles with a polymeric amide coating optimized for high resolution chromatography of complex glycan mixtures.

LudgerSep R2 HPLC column

LudgerSep R2 HPLC column

LS-R2-4.6x150

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Analysis by HPLC of 2-AA labeled monosaccharides in conjunction with the LudgerTag Quantitative Monosaccharide Analysis Kit (Catalogue No. LT-MONO-96).

LudgerSep uR2 UHPLC column – sialic acid

LudgerSep uR2 UHPLC column – sialic acid

LS-UR2-2.1x100

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Analysis of sialic acids labeled with 1,2-diamino-4,5 methylenedioxybenzene.2HCl (DMB) using UHPLC.

LudgerSep C3 anion exchange HPLC Column

LudgerSep C3 anion exchange HPLC Column

LS-C3-7.5x75

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Charged-based analysis and purification by HPLC of LudgerTag fluorophore and UV chromophore labeled glycans

LudgerSep N2 amide HPLC Column

LudgerSep N2 amide HPLC Column

LS-N2-2.0x150

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Analysis and purification by HPLC of LudgerTag fluorophore and UV-chromophore labeled glycans. LudgerSep™ N2 HPLC columns contain particles with a polymeric amide coating optimized for high resolution chromatography of complex glycan mixtures.

LudgerSep N2 amide HPLC Column

LudgerSep N2 amide HPLC Column

LS-N2-4.6x150

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Analysis and purification by HPLC of LudgerTag fluorophore and UV-chromophore labeled glycans. LudgerSep™ N2 HPLC columns contain particles with a polymeric amide coating optimized for high resolution chromatography of complex glycan mixtures.

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