Glycoprotein & glycopeptide
Glycoprotein & Glycopeptide
Glycoproteins are used as process controls for glycan release, and consecutive release followed by labelling. Glycoprotein standards that are widely available are IgG, mAbs and fetuin. These are useful during endoglycosidase release (N-glycans), hydrazinolysis, Orelea or beta elimination (generally O-glycans) and acid release (during monosaccharide/sialic acid analysis). Fetuin, IgG and mAbs can also be used as process controls for proteolytic digestion during glycopeptide mapping.
Glycopeptides are used as process controls for glycan release, and consecutive release followed by labelling. They are useful during both endoglycosidase release (N-glycans) and acid release (during monosaccharide/sialic acid analysis). They can also be used as purified standards during glycopeptide mapping.