N-Glycan - 2-AA labelled

N-Glycan  - 2-AA labelled

2-AA labeled glycans (2-aminobenzoic acid) are considered to be a superior standard for glycan analysis due to the higher sensitivity of detection as compared to 2-AB labeled glycans. Labeled glycans may be analyzed by high-sensitivity fluorescence detection or monitoring of UV-absorbance during various chromatographic and structure sequence analyses, as well as mass spectrometry. UHPLC, HPLC, ESI-MS, MALDI-MS and CE methods have been developed and are commonly used in the analysis of therapeutic glycoproteins such as EPO and antibody-based bio-therapeutics. Methods include ion exchange, normal phase, reverse phase, and size exclusion chromatography.

The 2-AA labeled glycans are supplied as 100 pmols dried form from an aqueous solution. They are stable at least 5 years as supplied and after reconstitution. Typically the 2-AA labeled glycans are reconstitute with 100 µls of the buffer solution being used, yielding a concentration of 1 pmol/µl. It is recommended to dissolve the dried glycans in the aqueous solution first (ammonium formate). 35ul + 65ul ACN will make 100 pmol in 100ul concentration.


Ludger N-Glycan Nomenclature Table

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N-Glycan Nomenclature Table


A1 glycan (A2G2S1, G2S1), 2-AA labelled

CAA-A1-01

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Biantennary N-glycan that contains one terminal sialic acid residue, 100pmol. m/z: 2052.7404

A2 glycan (A2G2S2, G2S2), 2-AA labelled

CAA-A2-01

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2-AA labeled disialylated biantennary oligosaccharide

A1F glycan (FA2G2S1, G2FS1), 2-AA labelled

CAA-A1F-01

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Biantennary N-glycan that contains one terminal sialic acid residue, and fucosylation on the core GlcNAc, 100pmol. m/z: 2198.7983

A2F glycan (FA2G2S2, G2FS2), 2-AA labelled

CAA-A2F-01

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Di-sialylated, core-fucosylated bi-antennary complex-type N-glycan (oligosaccharide).

A2G1 glycan (G1), 2AA labelled

CAA-A2G1-01

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Biantennary N-glycan that contains one terminal galactose residue. 

A3 glycan (A3G3S3), 2-AA labelled

CAA-A3-01

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2-AA labeled triantennary oligosaccharide. Triantennary N-glycan that contains terminal sialic acid residues, 100pmol. m/z: 3000.0634

FA2B glycan (G0B), 2-AA labelled

CAA-FA2B-01

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Biantennary N-glycan that contains terminal N-acetylglucosamine residues, a bisecting GlcNAc on the pentasaccharide core, and fucosylation on the core GlcNAc.

FA2BG1 glycan (G1B), 2-AA labelled

CAA-FA2BG1-01

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Biantennary N-glycan that contains one terminal galactose residue, a bisecting GlcNAc on the pentasaccharide core, and fucosylation on the core GlcNAc. Size: 100pmol.   m/z: 1989.7559

FA2G1 glycan (G1F), 2-AA labelled

CAA-FA2G1-01

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Biantennary N-glycan that contains one terminal galactose residue, and fucosylation on the core GlcNAc, Amount of sample:100pmol  m/z: 1745.6500

M3N2 (Man-3) glycan, 2-AA labelled

CAA-M3N2-01

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Oligomannose N-glycan with 3 mannose residues, Amount of sample:100pmol m/z: 1031.3805

Man-5 glycan, 2-AA labelled

CAA-MAN5-01

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Oligomannose N-glycan with 5 mannose residues, 100pmol. m/z: 1355.4862

Man-6 glycan, 2-AA labelled

CAA-MAN6-01

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Oligomannose N-glycan with 6 mannose residues, 100pmol.  m/z: 1517.5390 

Man-7 glycan, 2-AA labelled

CAA-MAN7-01

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Oligomannose N-glycan with 7 mannose residues, 100pmol.  m/z: 1679.5918

Man-8 glycan, 2-AA labelled

CAA-MAN8-01

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Oligomannose N-glycan with 8 mannose residues, 100pmol.  m/z: 1841.6447

Man-9 glycan, 2-AA labelled

CAA-MAN9-01

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Oligomannose N-glycan with 9 mannose residues, 100pmol. m/z: 2003.6975

NA2 glycan (A2G2, G2), 2-AA labelled

CAA-NA2-01

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Biantennary N-glycan that contains terminal galactose residues, 100pmol.   m/z: 1761.6449

NA2F glycan (FA2G2, G2F), 2-AA labelled

CAA-NA2F-01

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Biantennary N-glycan that contains terminal galactose residues and fucosylation on the core GlcNAc, 100pmol. m/z: 1907.7028

NA3 glycan (A3G3), 2-AA labelled

CAA-NA3-01

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Triantennary N-glycan that contains terminal galactose residues, 100pmol.  m/z: 2126.7771

NA4 glycan (A4G4), 2-AA labelled

CAA-NA4-01

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Tetraantennary N-glycan that contains terminal galactose residues, 100pmol.   m/z: 2491.9093

NGA2 glycan (A2, G0), 2-AA labelled

CAA-NGA2-01

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Biantennary N-glycan that contains terminal N-acetylglucosamine residues, 100pmol.   m/z: 1437.5393

NGA2F glycan (FA2, G0F), 2-AA labelled

CAA-NGA2F-01

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Biantennary N-glycan that contains terminal N-acetylglucosamine residues, and fucosylation on the core GlcNAc, 100pmol.  m/z: 1583.5972

NGA3 glycan (A3), 2-AA labelled

CAA-NGA3-01

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Triantennary N-glycan that contains terminal N-acetylglucosamine residues, 100pmol.  m/z: 1640.6787

NGA4 glycan (A4), 2-AA labelled

CAA-NGA4-01

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Tetraantennary N-glycan that contains terminal N-acetylglucosamine residues, 100pmol.   m/z: 1843.6980

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