Sialic Acid Profiling

Sialic Acid Profiling


The LudgerTag DMB Sialic Acid Labeling Kit has been developed for the qualitative analysis of sialic acids.

The kit contains reagents for the release of sialic acids from glycoproteins. Released sialic acids are conjugated with DMB dye by an amination-cyclisation reaction. The kit contains reagents and materials for up to 22 samples including the sialic acid reference panel, and the N-acetyl neuraminic acid and N-glycolyl neuraminic acid standards.

DMB Sialic Acid Labeling Kit are designed to satisfy the following regulatory requirements:

  • Overall degree of drug sialylation - absolute quantitation of sialic acid residues per molecule (nmol/mg protein).
  • Relative quantities of Neu5Ac:Neu5Gc.
  • Identification and relative percentage of O-acetylated sialic acids.

DMB sialic acid release & labelling kit

DMB sialic acid release & labelling kit

LT-KDMB-A1

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Release and labelling of sialic acids with 1,2-diamino-4,5-methylenedioxybenzene.2HCl (DMB) for fluorophore detection of chromatographically separated sialic acid variants. Kit sufficient for 22 samples.

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