Post-release Clean Up

Ludger EB10 cartidges have been designed for purification of glycans from proteins, salts, and detergents.

Cation exchange resin cartridges. For purification of O-glycans. Each pack contains 6 cartridges.

Ludger’s glycan purification PGC cartridges (catalogue no. LC-EC50-24) and plate (catalogue no. LC-EC50-96) have been designed to purify glycans from non-carbohydrate material including salts, proteins and detergents by electronic interaction of the glycans with the surface of the cartridge.

Each pack contains one 96-well clean-up plate. Optimised for removal of enzymes post-exoglycosidase digestion of glycans. For use in a vacuum manifold system (e.g. Ludger LV system) or centrifuge equipped with 96-well plate rotor.

The Ludger EC50 plates have been developed as a high throughput option to purify glycans from complex mixtures including proteins, salts, and detergents.

The Ludger Exoglycosidase Clean-up Cartridges are optimised for removal of enzymes post-exoglycosidase digestion of glycans. Use of these spin columns will prevent contamination of HPLC columns during subsequent chromatographic analysis.

Each pack contains one hydrophobic PVDF 96-well plate. For removal of enzymes and other proteins from unlabelled glycan mixtures. For use in a vacuum manifold system (e.g. Ludger LV system) or centrifuge equipped with 96-well plate rotor.

Each pack contains one hydrophilic interaction 96-well plate. Used for removal of excess salts, detergent, proteins and other impurities after the release of N-glycans from glycoproteins. This clean-up plate is used to enrich N-glycans prior to performing permethylation of released glycans.