Ludger EB10 cartidges have been designed for purification of glycans from proteins, salts, and detergents.
Applications include cleanup of glycans following hydrazinolysis, endoglycosidase digests (including PNGase F digests), enzyme treatment, and before and after fluorescent labeling.
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The Ludger EB10 cartridges contain a 10 mg bed of Ludger Electron Interaction Resin (EIR). This unique material contains very flat regions of pi-orbital electrons that interact with hydrophilic organic compounds including glycans.
The resin can capture a wide range of glycans from complex mixtures while salts and certain detergents pass through. The glycans are then eluted from the cartridge using a simple solvent system.
The cartridge housing is constructed of polypropylene and is compatible with many automated sample preparation systems.
Binding Capacity Each LudgerCleanTM EB10 cartridge can typically bind up to 50 μg of O- or N-linked glycans.
Number of Samples Ludger EB10 cartridges are designed for single use only.
Suitable Samples A wide range of glycans can be purified. These include N-linked and O-linked type oligosaccharides, tri-saccharides and larger structures.
The cartridges are not suitable either for monosaccharides or disaccharides which are generally bound too weakly for efficient purification or for large linear poly-sialylated glycans which can be bound very tightly to the resin.
Glycan samples must be applied to the cartridges in solutions that are substantially aqueous.
Structural Integrity No detectable (< 2 mole per cent) loss of sialic acid, fucose, sulfate, or phosphate.
Binding efficiency >95% for most glycans
Binding Selectivity Essentially stoichiometric binding and elution for most complex glycan mixtures.
Storage Store at room temperature in the dark. Protect from sources of heat, light, and moisture. The cartridges are stable for at least two years as supplied.
Shipping The product can be shipped at ambient temperature.
Handling Ensure that any glass, plastic ware or solvents used are free of glycosidases and environmental carbohydrates. Use powder-free gloves for all sample handling procedures and avoid contamination with environmental carbohydrate.
Safety Please read the Safety Data Sheet (SDS) for all chemicals used.
All processes involving hazardous reagents should be performed using appropriate personal safety protection – eyeglasses, chemically resistant gloves (e.g. nitrile), etc. – and where appropriate in a laboratory fume hood.