Exoglycosidases

Exoglycosidases

- Cleave specific terminal-monosacharides from glycans
- Allows detailed structural characterisation of glycans (monosaccharide type, linkage and sequence)
- All enzymes are tested for the absence of proteolytic or unexpected glycosidic activity

Example Enzyme Sequencing:

Workflow for exoglycosidase sequencing of glycans based on HPLC/LC-MS detection:


Ludger Exoglycosidase Sequencing Workflow

click to enlarge 


α-(1-2,3,6) mannosidase

α-(1-2,3,6) mannosidase

E-AM01

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Alpha-(1-2,3,6)-Mannosidase (Alpha-D-Mannoside Mannohydrolase)

α-(1,6) core mannosidase

α-(1,6) core mannosidase

E-AM02

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α(1-6) Core Mannosidase cleaves unbranched non-reducing terminal mannose, α(1-6) linked to the β-linked core mannose of the conserved mannosylchitobiose core of N-linked oligosaccharides. The presence of fucose linked to the core N-acetylglucosamine has no effect on cleavage.

α-(1-3,4) fucosidase

α-(1-3,4) fucosidase

E-F134

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α(1-3,4) Fucosidase cleaves branched non-reducing terminal fucose, linked α(1-3) or α(1-4) to the N-acetylglucosamine of terminal Gal-GlcNAc disaccharide structures. The presence of sialic acid (but not fucose) linked to the galactose will block cleavage.

β-(1-4)-galactosidase

β-(1-4)-galactosidase

E-BG07

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Non-reducing terminal β(1-4)-Galactose. Number of antennae does not affect cleavage rate. Fucose linked to the penultimate N-acetylglucosamine will block cleavage of the galactose.

β-N-acetylglucosaminidase

β-N-acetylglucosaminidase

E-GL01

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N-acetylglucosaminidase cleaves all non-reducing terminal β-linked N-acetylglucosamine residues from complex carbohydrates and glycoproteins.

Sialidase Au α-(2-3,6,8,9)

Sialidase Au α-(2-3,6,8,9)

E-S001

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α(2-3,6,8,9) Sialidase Au cleaves all cleaves all non-reducing terminal sialic acid residues from complex carbohydrates and glycoproteins. The relative cleavage rates for different linkages are: α(2-6) > α(2-3) > α(2-8), α(2-9).

Sialidase Cp α-(2-3,6)

Sialidase Cp α-(2-3,6)

E-S005

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α(2-3,6) Sialidase Cp cleaves all non-reducing terminal non-branched α(2-3) and α(2-6) sialic acid residues from complex carbohydrates and glycoproteins. There is no detectable activity on α(2-8) or α(2-9) linkages or on branched α(2-3) or α(2-6) linkages. The relative cleavage rates for different linkages are: α(2-3) > α(2-6).

Sialidase Sp α-(2-3)

Sialidase Sp α-(2-3)

E-S007

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Sialidase Sp cleaves the non-reducing terminal α(2-3) unbranched sialic acid residues from complex

Sialate O-acetylesterase kit

Sialate O-acetylesterase kit

LZ-ACASE-KIT

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To remove 9-, 8- and 7-O-acetyl groups from released sialic acids, released glycans or glycoproteins. Used for characterisation of highly sialylated biotherapeutics such as EPO, FSH and blood clotting factors. Kit includes enzyme plus reaction buffer. Sufficient for up to 50 samples.

LudgerZyme α(1-3,4) fucosidase kit

LudgerZyme α(1-3,4) fucosidase kit

LZ-FUCOSIDASE-01-KIT

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