Traditional labelling kits
Traditional labelling kits
LudgerTag Glycan Labeling Kit reagents are purified to analytical grade and are dispensed and sealed under clean, inert atmospheres. The ampoules are pre-cleaned by pyrolysis at 500°C then opened just before dispensing and sealing under oxygen-free dry nitrogen. These controls ensure that your analyses work properly each and every time.
2-AB and 2-AA are used in HILIC-(U)HPLC, WAX-HPLC, and MALDI-MS assays. They are the most referenced glycan label for biopharmaceutical analysis. 2-AA is also used for monosaccharide analysis.
Fluorescent labelling methods for glycoprofiling of therapeutic glycoproteins are well established and aid subsequent separation and quantitation using a range of techniques. These include high performance liquid chromatography (HPLC and UHPLC), capillary electrophoresis (CE), and mass spectrometry. Of the fluorescent labels available, 2-aminobenzamide (2-AB) and 2-aminobenzoic acid (2-AA) are most widely used for oligosaccharide profiling. Existing 2-AA and 2-AB labelling kits use sodium cyanoborohydride as a reducing agent during glycan labelling.