VTAG glycan release & labelling kit

VTAG glycan release & labelling kit


  • Product Code: LT-VTAG-C30
  • Size 30 samples

  • Enquire for Price

LudgerTag V-Tag for N-glycan Labelling

Ludger V-Tag Glycan Release and Labelling Kit

Our V-Tag glycan kit enables release of N-glycans from glycoproteins, N-glycan labelling and clean up within 1 hour for a single IgG type sample and 2 hours for 30 IgG type samples (timing for release by PNGase F might need to be adjusted for complex type samples). The glycans are then ready for analysis by (U)HPLC. Also, once labeled with V-Tag the glycans can be incubated with exoglycosidases to allow for structure assignment.

Each kit contains PNGase F, V-Tag fluorophore dye, reagents and SPE cartridges, sufficient for up to 30 samples.


Workflow:



Presentation Quick Links:

  1. Ludger V-Tag for N-glycan profiling and identification
  2. Highlights of the V-Tag System
  3. Validation Data
  4. Exoglycosidase Digestion Data
  5. Next Steps

 Downloadable pdf version of presentation


GlyShape presentation

We also have an additional presentation, as part of our GlyShape series, that introduces more broadly our V-Tag technology for N-glycan release and labelling. It includes a video that provides clear step-by-step instructions on how to perform the method using the LT-VTAG-C30 kit.

To view the presentation and for more information, please visit: www.ludger.com/glyshape


To enable release of N-glycans from glycoproteins, N-glycan labelling and clean up within 1 hour for a single sample / 2 hours for 30 samples. Each kit contains PNGase F, V-Tag fluorophore dye, reagents and SPE cartridges, sufficient for up to 30 samples.

LT-VTAG-C30 is used for the release of aminoglycans from glycoproteins, subsequent fluorophore labeling and clean up

This labelling kit contains the reagents needed for PNGase F release of aminoglycans, followed by fluorophore labeling and the specialised solid phase extraction (SPE) cartridges required for the purification of the labeled glycans.


Specifications for LT-VTAG-C30

Application: Release of aminoglycans from glycoproteins, subsequent fluorophore labeling and clean up

Description: This kit contains the reagents needed for PNGase F release of aminoglycans, followed by fluorophore labeling and the specialised solid phase extraction (SPE) cartridges required for the purification of the labeled glycans.

Number of Samples: 30 separate analytical samples per kit.

Amount of Sample: As a guideline up to 100 μg of glycoprotein per sample.

Suitable Samples: Glycoproteins and glycopeptides containing N-linked glycans.

Storage: Store at 4°C. Protect from sources of heat and light.

Shipping: The product should be shipped at 4°C.

Handling: Ensure that any glass, plastic ware or solvents used with this item are free of environmental carbohydrates and contaminating enzymes. Use powder-free gloves for all sample handling procedures and avoid contamination with environmental carbohydrate.

Safety: For research use only. Not for human or drug use . Please read the Safety Data Sheets (SDS's) for all chemicals used. All processes involving labeling reagents should be performed using appropriate personal safety protection – safety glasses, chemically resistant gloves (e.g. nitrile), lab coat, and when appropriate, in a laboratory fume cupboard.

Kit Contents


Handling: Ensure that any glass, plastic ware or solvents used with this item are free of environmental carbohydrates and contaminating enzymes. Use powder-free gloves for all sample handling procedures and avoid contamination with environmental carbohydrate.

Safety: For research use only. Not for human or drug use Please read the Safety Data Sheets (SDS's) for all chemicals used. All processes involving labeling reagents should be performed using appropriate personal safety protection – safety glasses, chemically resistant gloves (e.g. nitrile), lab coat, and when appropriate, in a laboratory fume cupboard.


Each labeling reaction set consists of one vial of each of the following: