Glycan Release

Glycan Release

A range of Ludger enzymes (Endoglycosidases and Exoglycosidases) and Ludger Liberate™ (chemical release) standalone products and kits are available at Ludger for release of N- and O-linked glycans from glycoprotein therapeutics, cell lines, biological fluids, or any glycoprotein moieties of interest to you.

Endoglycosidase F1

Endoglycosidase F1

E-EF01

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Endo F1 cleaves high mannose and some hybrid type N-glycans from peptides and proteins.

LudgerLiberate hydrazinolysis kit

LudgerLiberate hydrazinolysis kit

LL-HYDRAZ-A2

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The kit contains reagents for the release of N and O-linked glycans from glycoproteins via hydrazinolysis.

α-(1-2,3,6) mannosidase

α-(1-2,3,6) mannosidase

E-AM01

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Alpha-(1-2,3,6)-Mannosidase (Alpha-D-Mannoside Mannohydrolase)

Endoglycosidase F2

Endoglycosidase F2

E-EF02

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Endo F2 cleaves N-linked (asparagine-linked) biantennary oligosaccharides from glycoproteins. It also will cleave high mannose glycans but at a 40x reduced rate. It cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide, generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine. In contrast, PNGase F removes the oligosaccharide intact.

LudgerLiberate Orela kit

LudgerLiberate Orela kit

LL-ORELA-A2

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For release of O-linked glycans from glycoprotein therapeutics.

α-(1,6) core mannosidase

α-(1,6) core mannosidase

E-AM02

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α(1-6) Core Mannosidase cleaves unbranched non-reducing terminal mannose, α(1-6) linked to the β-linked core mannose of the conserved mannosylchitobiose core of N-linked oligosaccharides. The presence of fucose linked to the core N-acetylglucosamine has no effect on cleavage.

Endoglycosidase F3

Endoglycosidase F3

E-EF03

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Selective release of triantennarry and α(1-6) fucosylated biantennary N-glycans from peptides and protein.

α-(1-3,4) fucosidase

α-(1-3,4) fucosidase

E-F134

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α(1-3,4) Fucosidase cleaves branched non-reducing terminal fucose, linked α(1-3) or α(1-4) to the N-acetylglucosamine of terminal Gal-GlcNAc disaccharide structures. The presence of sialic acid (but not fucose) linked to the galactose will block cleavage.

β-(1-4)-galactosidase

β-(1-4)-galactosidase

E-BG07

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Non-reducing terminal β(1-4)-Galactose. Number of antennae does not affect cleavage rate. Fucose linked to the penultimate N-acetylglucosamine will block cleavage of the galactose.

Endoglycosidase H

Endoglycosidase H

E-EH02

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Endo H cleaves Asparagine-linked hybrid or high mannose oligosaccharides, but not complex oligosaccharides. It cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide, generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine. In contrast, PNGase F removes the oligosaccharide intact. Detergent and heat denaturation may increase the rate of cleavage for some glycoproteins.

O-glycosidase

O-glycosidase

E-G001

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O-glycosidase cleaves only unsubstituted Gal-β(1-3)GalNAc-α disaccharides attached to the serine or threonine residues of glycoproteins or glycopeptides.

β-N-acetylglucosaminidase

β-N-acetylglucosaminidase

E-GL01

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N-acetylglucosaminidase cleaves all non-reducing terminal β-linked N-acetylglucosamine residues from complex carbohydrates and glycoproteins.

PNGase F (Peptide-N-Glycosidase F)

PNGase F (Peptide-N-Glycosidase F)

E-PNG01

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PNGase F is suitable for release of all types (high-mannose, hybrid and complex) N-linked glycans from glycoproteins and glycopeptides. PNGase F will not remove oligosaccharides containing α(1-3) linked core fucose commonly found on plant glycoproteins.

Sialidase Au α-(2-3,6,8,9)

Sialidase Au α-(2-3,6,8,9)

E-S001

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α(2-3,6,8,9) Sialidase Au cleaves all cleaves all non-reducing terminal sialic acid residues from complex carbohydrates and glycoproteins. The relative cleavage rates for different linkages are: α(2-6) > α(2-3) > α(2-8), α(2-9).

PNGase F (Peptide N Glycosidase F)

PNGase F (Peptide N Glycosidase F)

E-PNG01-200

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PNGase F is suitable for release of all types (high-mannose, hybrid and complex) N-linked glycans from glycoproteins and glycopeptides. PNGase F will not remove oligosaccharides containing α(1-3) linked core fucose commonly found on plant glycoproteins.

Sialidase Cp α-(2-3,6)

Sialidase Cp α-(2-3,6)

E-S005

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α(2-3,6) Sialidase Cp cleaves all non-reducing terminal non-branched α(2-3) and α(2-6) sialic acid residues from complex carbohydrates and glycoproteins. There is no detectable activity on α(2-8) or α(2-9) linkages or on branched α(2-3) or α(2-6) linkages. The relative cleavage rates for different linkages are: α(2-3) > α(2-6).

Recombinant PNGase F

Recombinant PNGase F

E-rPNG01

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PNGase F is suitable for release of all types (high-mannose, hybrid and complex) N-linked glycans from glycoproteins and glycopeptides. PNGase F will not remove oligosaccharides containing α(1-3) linked core fucose commonly found on plant glycoproteins.

Sialidase Sp α-(2-3)

Sialidase Sp α-(2-3)

E-S007

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Sialidase Sp cleaves the non-reducing terminal α(2-3) unbranched sialic acid residues from complex

Endo-β-galactosidase

Endo-β-galactosidase

E-XBG01

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Endo-β-Galactosidase cleaves internal β(1-4) galactose linkages in unbranched, repeating poly-N-acetyllactosamine structures. Sulfated structures such as keratan sulfate are also cleaved. Branching and/or fucosylation of the substrate may decrease or eliminate cleavage.

Sialate O-acetylesterase kit

Sialate O-acetylesterase kit

LZ-ACASE-KIT

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To remove 9-, 8- and 7-O-acetyl groups from released sialic acids, released glycans or glycoproteins. Used for characterisation of highly sialylated biotherapeutics such as EPO, FSH and blood clotting factors. Kit includes enzyme plus reaction buffer. Sufficient for up to 50 samples.

Enzymatic CarboRelease kit

Enzymatic CarboRelease kit

KE-DG01

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This kit includes the enzymes and buffers required to deglycosylate glycoproteins, removing all N-linked oligosaccharides and many O-linked sugars.

LudgerZyme α(1-3,4) fucosidase kit

LudgerZyme α(1-3,4) fucosidase kit

LZ-FUCOSIDASE-01-KIT

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Enzymatic DeGlycoMx Kit

Enzymatic DeGlycoMx Kit

KE-DGMX

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This kit developed for protein deglycosylation includes our DeGlycoMx, a premixed cocktail of the enzymes required to remove all N-linked oligosaccharides and most O-linked sugars from 0.5 mg of glycoprotein, via 10 reactions of up to 50 micrograms of protein per reaction.

Ceramide Glycanase Kit

Ceramide Glycanase Kit

LZ-CER-HM-KIT

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Ceramide glycanase can be used to deglycosylate a variety of glycosphingolipids by cleaving the β-glycosyl linkage. Ceramide glycanase allows the identification of glycosylation patterns of glycosphingolipids through cleaving the glycan moiety and making it accessible to LudgerTag labelling technology.

LudgerZyme PNGase L Kit

LudgerZyme PNGase L Kit

LZ-PNGaseL-50-KIT

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Recombinant PNGase F

Recombinant PNGase F

LZ-rPNGaseF-kit

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LudgerZyme recombinant PNGase F kit (New England BioLabs).

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