Monosaccharide analysis is a regulatory requirement laid out in the ICH Q6B guidelines for characterisation of biopharmaceuticals. This information can be used at all stages of drug development as a method of determining the type of glycosylation (N-linked and/or O-linked) and the extent to which glycosylation has occurred. It can also be used to demonstrate consistency between batches for QC lot release during the manufacturing process.
Ludger’s monosaccharide analysis kit is easy to use, reliable and can be used for quantitative routine monosaccharide analysis. (Kit sufficient for 96 analyses).
- Release neutral and amino monosaccharides from your glycoprotein samples by mild acid hydrolysis
- Fluorescent labelling of released monosaccharides with 2-aminobenzoic acid (2AA)
- Relative quantitative analysis of 2AA-labelled monosaccharides compared to standards (GlcN, GalN, Gal, Man, Glc, Fuc, Xyl) by (U)HPLC analysis