Publications

2023


Ludger Publication - CVD biomarkers

Elevated concentrations of Neu5Ac and Neu5,9Ac2 in human plasma: potential biomarkers of cardiovascular disease

Jack Cheeseman, Concepcion Badia, Georgia Elgood-Hunt, Richard A. Gardner, Duong N. Trinh, Marco P. Monopoli, Gunter Kuhnle, Daniel I.R. Spencer & Helen M.I. Osborn.
Glycoconjugate Journal. 22 Nov 2023.
doi: 10.1007/s10719-023-10138-3


Ludger Publication - JCC

Serum N-Glycomic Biomarkers Predict Treatment Escalation in Inflammatory Bowel Disease

Archana Shubhakar, Bas C Jansen, Alex T Adams, Karli R Reiding, Nicholas T Ventham, Rahul Kalla, Daniel Bergemalm, Paulina A Urbanowicz, Richard A Gardner, IBD-BIOM Consortium , Jonas Halfvarson, Jack Satsangi, Daryl L Fernandes, Daniel I R Spencer.
Journal of Crohn's and Colitis. 24 Jan 2023.
doi: 10.1093/ecco-jcc/jjad012 -- PMID: 36694402

2022


Ludger Publication - IgE Characterisation

Generation and Characterization of Native and Sialic Acid-Deficient IgE

Alex J. McCraw, Richard A. Gardner, Anna M. Davies, Daniel I. R. Spencer, Melanie Grandits, Gerd K. Wagner ,James M. McDonnell, Sophia N. Karagiannis,Alicia Chenoweth, and Silvia Crescioli
International Journal of Molecular Sciences.
3 Nov 2022;23(21):13455.
doi: 10.3390/ijms232113455 PMID: 36362241
Free PMC article

Ludger Publication - Plant Breakdown

Plant N-glycan breakdown by human gut Bacteroides

Lucy I. Crouch, Paulina A. Urbanowicz, Arnaud Baslé, Zhi-Peng Cai, Li Liu, Josef Voglmeir, Javier M Melo Diaz, Samuel T Benedict, Daniel I R Spencer, David N Bolam
Proc Natl Acad Sci USA. 27 Sep 2022;119(39):e2208168119.
doi: 10.1073/pnas.2208168119 Epub 19 Sep 2022. PMID: 36122227
Free PMC article

Ludger Publication - Corona Cholesterol

Biomolecular Corona Stability in Association with Plasma Cholesterol Level

Trinh DN, Radlinskaite M, Cheeseman J, Kuhnle G, Osborn HMI, Meleady P, Spencer DIR, Monopoli MP
Nanomaterials (Basel). 3 Aug 2022;12(15):2661
doi: 10.3390/nano12152661 PMID: 35957093
Free PMC article

Ludger Publication - Egg yolk

Egg yolk sialylglycopeptide: purification, isolation and characterization of N-glycans from minor glycopeptide species

Javier Mauricio Melo Diaz, Alan B. Moran, Simon R. Peel, Jenifer L. Hendel, and Daniel I. R. Spencer
Organic and Biomolecular Chemistry Royal Society of Chemistry Journal. 16 May 2022.
doi: 10.1039/d2ob00615d

Ludger Publication - Nanoparticle Corona

Probing the glycans accessibility in the nanoparticle biomolecular corona

Clemente E, Martinez-Moro M, Trinh DN, Soliman MG, Spencer DIR, Gardner RA, Kotsias M, Sánchez Iglesias A, Moya S, Monopoli MP
J Colloid Interface Sci. May 2022;613:563-574.
doi: 10.1016/j.jcis.2021.11.140 Epub 16 Dec 2022 -- PMID: 35066229

Ludger Publication - Tannerella

Structural and functional characterisation of a stable, broad-specificity multimeric sialidase from the oral pathogen Tannerella forsythia

Satur MJ, Urbanowicz PA, Spencer DIR, Rafferty J, Stafford GP
Biochem J.. 16 Sep 2022;479(17):1785-1806
doi: 10.1042/BCJ20220244 PMID: 35916484
Free PMC article

Ludger Publication - Beans

Extraction and purification of a High Mannose type oligosaccharide from Phaseolus lunatus beans by oxidative release with sodium hypochlorite

Melo Diaz JM, Peel SR, Spencer DIR, Hendel JL
Carbohydr Res. Jul 2022;517:108583
doi: 10.1016/j.carres.2022.108583 Epub May 2022. -- PMID: 35569240

Ludger Publication - Linkage Differenciation

Sialic Acid Derivatization of Fluorescently Labeled N-Glycans Allows Linkage Differentiation by Reversed-Phase Liquid Chromatography-Fluorescence Detection-Mass Spectrometry

Moran AB, Gardner RA, Wuhrer M, Lageveen-Kammeijer GSM, Spencer DIR
Anal Chem. 10 May 2022;;94(18):6639-6648.
doi: 10.1021/acs.analchem.1c02610 Epub 2022 Apr 28 -- PMID: 35482581
Free PMC article

Ludger Publication - Nanoparticles Biomarker

Nanoparticle Biomolecular Corona-Based Enrichment of Plasma Glycoproteins for N-Glycan Profiling and Application in Biomarker Discovery

Trinh DN, Gardner RA, Franciosi AN, McCarthy C, Keane MP, Soliman MG, O'Donnell JS, Meleady P, Spencer DIR, Monopoli MP.
ACS Nano. 2022. 26 Apr 2022;16(4):5463-5475.
doi: 10.1021/acsnano.1c09564
Free PMC article

Ludger Publication - Glycobiology

Development of an exoglycosidase plate-based assay for detecting α1-3,4 fucosylation biomarker in individuals with HNF1A-MODY

Demus D, Urbanowicz PA, Gardner RA, Wu H, Juszczak A, Štambuk T, Medvidović EP, Owen KR, Gornik O, Juge N, Spencer DIR
Glycobiology. 30 Mar 2022;32(3):230-238
doi: 10.1093/glycob/cwab107 -- PMID: 34939081
Free PMC article

Ludger Publication - Macrophage Protection

Sialylation on O-linked glycans protects von Willebrand factor from macrophage galactose lectin-mediated clearance

DWard SE, O'Sullivan JM, Moran AB, Spencer DIR, Gardner RA, Sharma J, Fazavana J, Monopoli M, McKinnon TAJ, Chion A, Haberichter S, O'Donnell JS
Haematologica. 1 Mar 2022;;107(3):668-679
doi: 10.3324/haematol.2020.274720 -- PMID: 33763999 -- PMCID: PMC8883566
Free PMC article

Ludger Publication - ChemBioChem

Quantitative Standards of 4-O-Acetyl- and 9-O-Acetyl-N-Acetylneuraminic Acid for the Analysis of Plasma and Serum

Cheeseman J, Badia C, Thomson RI, Kuhnle G, Gardner RA, Spencer DIR, Osborn HMI
ChemBioChem. 4 Mar 2022;23(5):e202100662.
doi: 10.1002/cbic.202100662 Epub 18 Jan 2022 -- PMID: 34874597
Free PMC article

2021


Ludger Publication - Antibodies

Glycoengineering of Therapeutic Antibodies with Small Molecule Inhibitors

Li S, McCraw AJ, Gardner RA, Spencer DIR, Karagiannis SN, Wagner GK
Antibodies. 4 Nov 2021.
doi: 10.3390/antib10040044 -- PMID: 34842612

Ludger Publication - Antibody Glycosylation - Experientia Supplementum book series

Automation of Immunoglobulin Glycosylation Analysis

Hendel JL, Gardner RA, Spencer DIR
Antibody Glycosylation - Experientia Supplementum Book Series. 23 Oct 2021.
doi: 10.1007/978-3-030-76912-3_5 -- PMID: 34687010

Ludger Publication - Journal of Biotechnology

Expression and characterization of a novel single-chain anti-vascular endothelial growth factor antibody in the goat milk

Leiva MJ, Parra NC, Contreras MA, KCS Tavares, Macaya L, Martins L, Gaudencio S, Hidalgo A, Kozak RP, González A, Sánchez O, Toledo JR, Montesino R
Journal of Biotechnology. 10 Sep 2021.
doi: 10.1016/j.jbiotec.2021.06.025 -- PMID: 34224759

Ludger Publication - Biomarkers in Medicine

Sialic acid as a potential biomarker for cardiovascular disease, diabetes and cancer

Cheeseman J, Kuhnle G, Stafford G, Gardner RA, Spencer DIR, Osborn HM
Biomarkers in Medicine. Aug 2021.
doi: 10.2217/bmm-2020-0776 -- PMID: 34241547

Ludger Publication - Glycoconjugate Journal

A semi-automated, high throughput approach for O-glycosylation profiling of in vitro established cancer cell lines by MALDI-FT-ICR MS

Kotsias M, Madunić K, Nicolardi S, Kozak RP, Gardner RA, Jansen BC, Spencer DIR, Wuhrer M
Glycoconjugate Journal. 20 Jul 2021.
doi: 10.1007/s10719-021-10003-1 -- PMID: 34283362

Ludger Publication - Glycoconjugate Journal

Interlaboratory evaluation of plasma N-glycan antennary fucosylation as a clinical biomarker for HNF1A-MODY using liquid chromatography methods

Demus D, Jansen BC, Gardner RA, Urbanowicz PA, Wu H, Štambuk T, Juszczak A, Medvidović EP, Juge N, Gornik O, Owen KR, Spencer DIR
Glycoconjugate Journal. 25 Mar 2021.
doi: 10.1007/s10719-021-09992-w -- PMID: 33765222

Ludger Publication - PLOS Neglected Tropical Diseases
Photo credit: Oregon State University © 2011

Tsetse salivary glycoproteins are modified with paucimannosidic N-glycans, are recognised by C-type lectins and bind to trypanosomes

Kozak RP, Mondragon-Shem K, Williams C, Rose C, Perally S, Caljon G, Van Den Abbeele J, Wongtrakul-Kish K, Gardner RA, Spencer D, Lehane MJ, Acosta-Serrano A
PLOS Neglected Tropical Diseases. 2 Feb 2021.
doi: 10.1371/journal.pntd.0009071 -- PMID: 33529215


2020


Ludger Publication - Nature Communications

Prominent members of the human gut microbiota express endo-acting O-glycanases to initiate mucin breakdown

Crouch LI, Liberato MV, Urbanowicz PA, Baslé A, Lamb CA, Stewart CJ, Cooke K, Doona M, Needham S, Brady RR, Berrington JE, Madunic K, Wuhrer M, Chater P, Pearson JP, Glowacki R, Martens EC, Zhang F, Linhardt RJ, Spencer DIR, Bolam DN
Nature Communications. 11 Aug 2020.
doi: 10.1038/s41467-020-17847-5 -- PMID: 32782292

Ludger Publication - Scientific Reports

Insights into the salivary N‑glycome of Lutzomyia longipalpis, vector of visceral leishmaniasis

Mondragon-Shem K, Wongtrakul-Kish K, Kozak RP, Yan S, Wilson IBH, Paschinger K, Rogers ME, Spencer DIR, Acosta-Serrano A.
Scientific Reports. 31 Jul 2020.
doi:10.1038/s41598-020-69753-x

Ludger publication - Microbiology

Fucosidases from the human gut symbiont Ruminococcus gnavus

Wu H, Rebello O, Crost EH, David Owen C, Walpole S, Bennati-Granier C, Ndeh D, Monaco S, Hicks T, Colvile A, Urbanowicz PA, Walsh MA, Angulo J, Spencer DIR, Juge N.
Cellular and Molecular Life Sciences. Apr 2020.
doi:10.1007/s00018-020-03514-x

Ludger publication - Frontiers in Chemistry

A Matrix-Assisted Laser Desorption/Ionization-Mass Spectrometry Assay for the Relative Quantitation of Antennary Fucosylated N-Glycans in Human Plasma

Rebello OD, Nicolardi S, Lageveen-Kammeijer GSM, Nouta J, Gardner RA, Mesker WE, Tollenaar RAEM, Spencer DIR, Wuhrer M, Falck D.
Frontiers in Chemistry. 28 Feb 2020.
doi: 10.3389/fchem.2020.00138


2019


Ludger publication - Microbiology

Characterization of Porphyromonas gingivalis sialidase and disruption of its role in host-pathogen interactions

Frey AM, Satur MJ, Phansopa C, Honma K, Urbanowicz PA, Spencer DIR, Pratten J, Bradshaw D, Sharma A, Stafford G.
Microbiology. Nov 2019.
doi: 10.1099/mic.0.000851

Ludger publication - Complex N-glycan breakdown by gut Bacteroids

Complex N-glycan breakdown by gut Bacteroides involves an extensive enzymatic apparatus encoded by multiple co-regulated genetic loci

Briliūtė J, Urbanowicz PA, Luis AS, Baslé A, Paterson N, Rebello O, Hendel J, Ndeh DA, Lowe EC, Martens EC, Spencer DIR, Bolam DN, Crouch LI.
Nature Microbiology. 2019 Jun 3.
doi: 10.1038/s41564-019-0466-x

Ludger RSC Synthetic Glycomes Chapter

Current Stage of Commercially Available Glycans to Support Realization of Biologic Drugs

Hendel JL, Fernandes DL.
Synthetic Glycomes. Chapter 17. P393-420. Edited by Guan W, Li L and Wang PG, London: The Royal Society of Chemistry 2019.
doi:10.1039/9781788016575-00393

Improved and semi-automated reductive β-elimination workflow for higher throughput protein O-glycosylation analysis.

Kotsias M, Kozak RP, Gardner RA, Wuhrer M, Spencer DIR.
PLoS One. 2019;14(1):e0210759. Published 2019 Jan 17.
doi:10.1371/journal.pone.0210759


2018


Transcriptional activation of fucosyltransferase (FUT) genes using the CRISPR-dCas9-VPR technology reveals potent N-glycome alterations in colorectal cancer cells.

Blanas A, Cornelissen LAM, Kotsias M, van der Horst JC, van de Vrugt HJ, Kalay H, Spencer DIR, Kozak RP, van Vliet SJ.
Glycobiology. 2018 Nov 22.
doi: 10.1093/glycob/cwy096

High-throughput serum N-glycomics: method comparison and application to study rheumatoid arthritis and pregnancy-associated changes.

Reiding KR, Bondt A, Hennig R, Gardner RA, O'Flaherty R, Trbojevic-Akmacic I, Shubhakar A, Hazes J, Reichl U, Fernandes DL, Pucic-Bakovic M, Rapp E, Spencer DIR, Dolhain R, Rudd P, Lauc G, Wuhrer M.
Molecular & Cellular Proteomics. 2018 Sep 21. pii: mcp.RA117.000454.
doi: 10.1074/mcp.RA117.000454

GlycoStore: a database of retention properties for glycan analysis.

Zhao S, Walsh I, Abrahams JL, Royle L, Nguyen-Khuong T, Spencer D, Fernandes DL, Packer NH, Rudd PM, Campbell MP.
Bioinformatics. 2018 Sep 15;34(18):3231-3232.
doi: 10.1093/bioinformatics/bty319

HappyTools: A software for high-throughput HPLC data processing and quantitation.

Jansen BC, Hafkenscheid L, Bondt A, Gardner RA, Hendel JL, Wuhrer M, Spencer DIR.
PLoS One. 2018 Jul 6;13(7):e0200280.
doi: 10.1371/journal.pone.0200280

Towards automation of glycomic profiling of complex biological materials.

Shubhakar A, Pang PC, Fernandes DL, Dell A, Spencer DIR, Haslam SM.
Glycoconjugate Journal. 2018 Jun 16.
doi: 10.1007/s10719-018-9825-8

Ludger publication - Engineering and stable production of recombinant IgE for cancer immunotherapy and AllergoOncology

Engineering and stable production of recombinant IgE for cancer immunotherapy and AllergoOncology.

Crescioli S, Chiaruttini G, Mele S, Ilieva KM, Pellizzari G, Spencer DIR, Gardner RA, Lacy KE, Spicer JF, Tutt ANJ, Wagner GK, Karagiannis SN.
Journal of Allergy and Clinical Immunology. 2018 Apr;141(4):1519-1523.e9.
doi: 10.1016/j.jaci.2017.12.986


2017


Ludger publication - Analysis of Three Epoetin Alpha Products by LC and LC-MS

Analysis of Three Epoetin Alpha Products by LC and LC-MS Indicates Differences in Glycosylation Critical Quality Attributes, Including Sialic Acid Content.

Thomson RI, Gardner RA, Strohfeldt K, Fernandes DL, Stafford GP, Spencer DIR, Osborn HMI.
Analytical Chemistry. 2017 Jun 20;89(12):6455-6462.
doi: 10.1021/acs.analchem.7b00353


2016


Ludger publication - Variation of Human Salivary O-Glycome

Variation of Human Salivary O-Glycome.

Kozak RP, Urbanowicz PA, Punyadeera C, Reiding KR, Jansen BC, Royle L, Spencer DI, Fernandes DL, Wuhrer M.
PLoS One. 2016 Sep 9;11(9):e0162824.
doi: 10.1371/journal.pone.0162824

Automated High-Throughput Permethylation for Glycosylation Analysis of Biologics Using MALDI-TOF-MS.

Shubhakar A, Kozak RP, Reiding KR, Royle L, Spencer DI, Fernandes DL, Wuhrer M.
Analytical Chemistry. 2016 Sep 6;88(17):8562-9.
doi: 10.1021/acs.analchem.6b01639

Composition and Antigenic Effects of Individual Glycan Sites of a Trimeric HIV-1 Envelope Glycoprotein.

Behrens AJ, Vasiljevic S, Pritchard LK, Harvey DJ, Andev RS, Krumm SA, Struwe WB, Cupo A, Kumar A, Zitzmann N, Seabright GE, Kramer HB, Spencer DI, Royle L, Lee JH, Klasse PJ, Burton DR, Wilson IA, Ward AB, Sanders RW, Moore JP, Doores KJ, Crispin M.
Cell Reports. 2016 Mar 22;14(11):2695-706.
doi: 10.1016/j.celrep.2016.02.058

Longitudinal monitoring of immunoglobulin A glycosylation during pregnancy by simultaneous MALDI-FTICR-MS analysis of N- and O-glycopeptides.

Bondt A, Nicolardi S, Jansen BC, Stavenhagen K, Blank D, Kammeijer GS, Kozak RP, Fernandes DL, Hensbergen PJ, Hazes JM, van der Burgt YE, Dolhain RJ, Wuhrer M.
Scientific Reports. 2016 Jun 15;6:27955.
doi: 10.1038/srep27955


2015


Characterization of a sialate-O-acetylesterase (NanS) from the oral pathogen Tannerella forsythia that enhances sialic acid release by NanH, its cognate sialidase.

Phansopa C, Kozak RP, Liew LP, Frey AM, Farmilo T, Parker JL, Kelly DJ, Emery RJ, Thomson RI, Royle L, Gardner RA, Spencer DI, Stafford GP.
Biochemical Journal. 2015 Dec 1;472(2):157-67.
doi: 10.1042/BJ20150388

Parasite Glycobiology: A Bittersweet Symphony.

Rodrigues JA, Acosta-Serrano A, Aebi M, Ferguson MA, Routier FH, Schiller I, Soares S, Spencer D, Titz A, Wilson IB, Izquierdo L.
PLoS Pathogens. 2015 Nov 12;11(11):e1005169.
doi: 10.1371/journal.ppat.1005169

Glycan Microheterogeneity at the PGT135 Antibody Recognition Site on HIV-1 gp120 Reveals a Molecular Mechanism for Neutralization Resistance.

Pritchard LK, Spencer DI, Royle L, Vasiljevic S, Krumm SA, Doores KJ, Crispin M.
Journal of Virology. 2015 Jul;89(13):6952-9.
doi: 10.1128/JVI.00230-15

Glycan clustering stabilizes the mannose patch of HIV-1 and preserves vulnerability to broadly neutralizing antibodies.

Pritchard LK, Spencer DI, Royle L, Bonomelli C, Seabright GE, Behrens AJ, Kulp DW, Menis S, Krumm SA, Dunlop DC, Crispin DJ, Bowden TA, Scanlan CN, Ward AB, Schief WR, Doores KJ, Crispin M.
Nature Communications. 2015 Jun 24;6:7479.
doi: 10.1038/ncomms8479

Development of a Schistosoma mansoni shotgun O-glycan microarray and application to the discovery of new antigenic schistosome glycan motifs.

van Diepen A, van der Plas AJ, Kozak RP, Royle L, Dunne DW, Hokke CH.
International Journal for Parasitology. 2015 Jun;45(7):465-75.
doi: 10.1016/j.ijpara.2015.02.008

Changes to serum sample tube and processing methodology does not cause Intra-Individual [corrected] variation in automated whole serum N-glycan profiling in health and disease.

Ventham NT, Gardner RA, Kennedy NA, Shubhakar A, Kalla R, Nimmo ER; IBD-BIOM Consortium, Fernandes DL, Satsangi J, Spencer DI.
PLoS One. 2015 Apr 1;10(4):e0123028.
doi: 10.1371/journal.pone.0123028

High-Throughput Analysis and Automation for Glycomics Studies.

Shubhakar A, Reiding KR, Gardner RA, Spencer DI, Fernandes DL, Wuhrer M.
Chromatographia. 2015;78(5-6):321-333.
doi: 10.1007/s10337-014-2803-9

Mass spectrometry for glycosylation analysis of biopharmaceuticals.

Dotz V, Haselberg R, Shubhakar A, Kozak RP, Falck D, Rombouts Y, Reusch D, Somsen GW, Fernandes DL, Wuhrer M.
TrAC Trends in Analytical Chemistry. Volume 73, November 2015, Pages 1-9.
doi: 10.1016/j.trac.2015.04.024


2014


The role of glycosylation in IBD.

Theodoratou E, Campbell H, Ventham NT, Kolarich D, Pučić-Baković M, Zoldoš V, Fernandes D, Pemberton IK, Rudan I, Kennedy NA, Wuhrer M, Nimmo E, Annese V, McGovern DP, Satsangi J, Lauc G.
Nature Reviews Gastroenterology & Hepatology. 2014 Oct;11(10):588-600.
doi: 10.1038/nrgastro.2014.78

Carbohydrate sequence of the prostate cancer-associated antigen F77 assigned by a mucin O-glycome designer array.

Gao C, Liu Y, Zhang H, Zhang Y, Fukuda MN, Palma AS, Kozak RP, Childs RA, Nonaka M, Li Z, Siegel DL, Hanfland P, Peehl DM, Chai W, Greene MI, Feizi T.
Journal of Biological Chemistry. 2014 Jun 6;289(23):16462-77.
doi: 10.1074/jbc.M114.558932

Improved nonreductive O-glycan release by hydrazinolysis with ethylenediaminetetraacetic acid addition.

Kozak RP, Royle L, Gardner RA, Bondt A, Fernandes DL, Wuhrer M.
Analytical Biochemistry. 2014 May 15;453:29-37.
doi: 10.1016/j.ab.2014.02.030

Mutations in B4GALNT1 (GM2 synthase) underlie a new disorder of ganglioside biosynthesis.

Harlalka GV, Lehman A, Chioza B, Baple EL, Maroofian R, Cross H, Sreekantan-Nair A, Priestman DA, Al-Turki S, McEntagart ME, Proukakis C, Royle L, Kozak RP, Bastaki L, Patton M, Wagner K, Coblentz R, Price J, Mezei M, Schlade-Bartusiak K, Platt FM, Hurles ME, Crosby AH.
Brain: A Journal of Neurology. 2013 Dec;136(Pt 12):3618-24.
doi: 10.1093/brain/awt270


2013


Effect of a tail piece cysteine deletion on biochemical and functional properties of an epidermal growth factor receptor-directed IgA2m(1) antibody.

Brunke C, Lohse S, Derer S, Peipp M, Boross P, Kellner C, Beyer T, Dechant M, Royle L, Liew LP, Leusen JH, Valerius T.
mAbs. 2013 Nov-Dec;5(6):936-45.
doi: 10.4161/mabs.26396


2012


Suppression of peeling during the release of O-glycans by hydrazinolysis.

Kozak RP, Royle L, Gardner RA, Fernandes DL, Wuhrer M.
Analytical Biochemistry. 2012 Apr 1;423(1):119-28.
doi: 10.1016/j.ab.2012.01.002

Protein O-glycosylation analysis.

Zauner G, Kozak RP, Gardner RA, Fernandes DL, Deelder AM, Wuhrer M.
Biological Chemistry. 2012 Aug;393(8):687-708.
doi: 10.1515/hsz-2012-0144