Monosaccharide and Sialic Acid Standards

Monosaccharide Standards

Quantitative monosaccharide standard

CM-MONOMIX-10

Mix of six monosaccharide quantitative standards (glucosamine, galactosamine, galactose, mannose, glucose(dextrose) and fucose), 10nmols of each.

This component is also part of our LudgerTag Quantitative Monosaccharide Release and 2-AA (2-aminobenzoic acid) Labelling Kit (LT-MONO-96)

CM-MONOMIX-10X3

Mix of six monosaccharide quantitative standards (glucosamine, galactosamine, galactose, mannose, glucose(dextrose) and fucose)

10nmols of each, pack of 3

Figure 1: 2AA-labeled monosaccharide standards profiled on a LudgerSep uR2 UHPLC column (Cat No. LS-UR2-2.1x50). Peaks for the following monosaccharides appear within 8 minutes; glucosamine (GlcN), galactosamine (GalN), galactose (Gal), mannose (Man), glucose (Glc), xylose (Xyl) and fucose (Fuc)

Quantitative xylose standard

CM-XYLOSE-100

Xylose quantitative standard, 100nmols

Mannose 6 phosphate standard

CM-MAN6P-10

Mannose 6 phosphate quantitative standard, 10nmols

Sialic Acid Standards

N-glycolylneuraminic acid (Neu5Gc or NGNA) quantitative standards

CM-NEU-GC-01

N -glycolylneuraminic acid (Neu5Gc or NGNA) quantitative standard, 1 nmol

CM-NEUGC-100

N -glycolylneuraminic acid (Neu5Gc or NGNA) quantitative standard, 100 nmol

N-acetylneuraminic acid (Neu5Ac or NANA) quantitative standards

CM-NEU-AC-01

N -acetylneuraminic acid (Neu5Ac or NANA) quantitative standard, 1 nmol

CM-NEUAC-100

N -acetylneuraminic acid (Neu5Ac or NANA) quantitative standard, 100 nmol

5-N-acetyl-9-O-acetyl neuraminic acid standard (Neu5,9Ac2)

CM-NEU5,9AC2-01

This sialic acid standard is a mixture of various sialic acids. The main sialic acid species present, 5-N-acetyl-9-O-acetyl neuraminic acid (Neu5,9Ac2), makes up 77 % of the mixture, 1nmol.

This product is only for use as a chromatography standard and should be not used as a quantitative standard.

Sialic acid reference panel

CM-SRP-01

The sialic acid reference panel is a mixture of different sialic acid forms sourced from bovine submaxillary mucin.

The panel contains seven sialic acids which include: Neu5Ac, Neu5Gc, Neu5,7Ac2, Neu5Gc,9Ac, Neu5,8Ac2, Neu5,9Ac2 and Neu5,x,xAc3 (where x is an unknown acetyl position). Peak assignment in the chromatogram ( Figure 1) is based on literature, standard inclusion, and NMR.

CM-SRP-01 panel is a standalone product as well as a component in the LudgerTag DMB sialic acid release and labelling kit (LT-KDMB-A1). This kit and the panel can assist you in performing sialic acid analysis which is a regulatory requirement to monitor the level and types of sialic acids during all stages of biopharmaceutical realisation.

Figure 1: LudgerSep R1 HPLC profile of DMB labelled Sialic acid Reference Panel

Visit our page Ludger BioQuant standards for more quantitative standards
(BQ-GPEP-A2G2S2-10U, BQ-CHITOTRIOSE-01, BQ-CN-MAN8-10U, BQ-CAB-CHI-01, BQ-CAA-CHI-01)