Exoglycosidases:

- Cleave specific terminal-monosacharides from glycans
- Allows detailed structural characterisation of glycans (monosaccharide type, linkage and sequence)
- All enzymes are tested for the absence of proteolytic or unexpected glycosidic activity


Example Enzyme Sequencing:


Ludger Enzyme Sequencing Example





  • Sialidase Au α(2-3,6,8,9)


    Ludger Sialidase Au enzyme structure

    E-S001

    α(2-3,6,8,9) Sialidase cleaves all non-reducing terminal sialic acid residues from complex carbohydrates and glycoproteins. In addition, the enzyme will cleave branched sialic acids (linked to an internal residue).

    Recombinant from Arthrobacter ureafaciens in E. coli


    View product documentation: Specsheet / CofA / MSDS

    • Part Number
    • E-S001
    • Amount of Enzyme
    • 0.3 U / 60 µL






    Kit includes enzyme plus reaction buffer.

    Sufficient for up to 60 reactions.


  • Sialidase Cp α(2-3,6)


    Ludger Sialidase Cp enzyme structure

    E-S005

    Sialidase Cp cleaves all non-reducing terminal non-branched α(2-3) and α(2-6) sialic acid residues from complex carbohydrates and glycoproteins.

    Recombinant from Clostridium perfringens in E. coli


    View product documentation: Specsheet / CofA / MSDS

    • Part Number
    • E-S005
    • Amount of Enzyme
    • 0.9 U / 60 µL






    Kit includes enzyme plus reaction buffer.

    Sufficient for up to 60 reactions.


  • Sialidase Sp α(2-3)


    Ludger Sialidase Sp enzyme structure

    E-S007

    Sialidase Sp cleaves the non-reducing terminal α(2-3) unbranched sialic acid residues from complex carbohydrates and glycoproteins.

    Recombinant from Streptococcus pneumoniae in E. Coli


    View product documentation: Specsheet / CofA / MSDS

    • Part Number
    • E-S007
    • Amount of Enzyme
    • 0.3 U / 60 µL






    Kit includes enzyme plus reaction buffer.

    Sufficient for up to 60 reactions.


  • LudgerZyme Acetyl esterase (sialate-O-acetylesterase) kit


    Ludger Acetyl Esterase enzyme structure

    LZ-ACASE-KIT

    To remove 9-, 8- and 7-O-acetyl groups from released sialic acids, released glycans or glycoproteins. Used for characterisation of highly sialylated biotherapeutics such as EPO, FSH and blood clotting factors.

    Recombinant from Tannerella forsythia in E. Coli.


    Product guide

    Certificate of stability

    Certificates of analysis:   Batch # B617-04

    • Part Number
    • LZ-ACASE-KIT
    • Amount of Enzyme
    • 50 µL






    Kit includes enzyme plus reaction buffer.

    Sufficient for up to 50 samples.


  • β(1-4)-galactosidase


    Ludger Beta-(1-4)-galactosidase enzyme structure

    E-BG07

    Non-reducing terminal β(1-4)-Galactose. Number of antennae does not affect cleavage rate. Fucose linked to the penultimate N-acetylglucosamine will block cleavage of the galactose.

    Recombinant from Streptococcus pneumoniae in E. coli


    View product documentation: Specsheet / CofA / MSDS

    • Part Number
    • E-BG07
    • Amount of Enzyme
    • 0.18 U / 60 µL






    Kit includes enzyme plus reaction buffer.

    Sufficient for up to 60 reactions.


  • β(1-3,4,6)-galactosidase


    Ludger Beta-(1-3,4,6)-galactosidase enzyme structure

    E-BG02

    Cleaves all β1-3 and β1-4 linked non-reducing, terminal galactose. β1-6 linked galactose is released at a slower rate.

    Bovine testes


    View product documentation: Specsheet / CofA / MSDS

    • Part Number
    • E-BG02
    • Amount of Enzyme
    • 0.5 U / 200 µL






    Kit includes enzyme plus reaction buffer.

    Sufficient for up to 200 reactions.


  • α(1-3,6)-galactosidase


    Ludger Alpha-(1-3,6)-galactosidase enzyme structure

    E-AG02    *formerly E-AG01

    Alpha Galactosidase from E. coli cleaves α(1-3) and α(1-6) linked, non-reducing terminal galactose from complex carbohydrates and glycoproteins.

    Recombinant from E. coli in E. coli


    View product documentation: Specsheet / CofA / MSDS

    • Part Number
    • E-AG02
    • Amount of Enzyme
    • 0.4 U / 60 µL






    Kit includes enzyme plus reaction buffer.

    Sufficient for up to 60 reactions.


  • β(1-2,3,4,6)-N-acetylglucosaminidase


    Ludger N-acetylglucosaminidase enzyme structure

    E-GL01

    N-acetylglucosaminidase cleaves all non-reducing terminal β-linked N-acetylglucosamine residues from complex carbohydrates and glycoproteins.

    Recombinant from Streptococcus pneumoniae in E. coli


    View product documentation: Specsheet / CofA / MSDS

    • Part Number
    • E-GL01
    • Amount of Enzyme
    • 2.4 U / 60 µL






    Kit includes enzyme plus reaction buffer.

    Sufficient for up to 60 reactions.


  • α(1-2,3,6)-mannosidase


    Ludger Alpha-(1-2,3,6)-mannosidase enzyme structure

    E-AM01

    Cleaves all α(1-2,3,6) linked mannose

    From Jack Bean


    View product documentation: Specsheet / CofA / MSDS

    • Part Number
    • E-AM01
    • Amount of Enzyme
    • 0.6 U / 60 µL






    Kit includes enzyme plus reaction buffer.

    Sufficient for up to 60 reactions.


  • α(1,6) core mannosidase


    Ludger Alpha-(1,6) core mannosidase enzyme structure

    E-AM02

    α(1-6) core mannosidase cleaves unbranched non-reducing terminal mannose, α(1-6) linked to the β-linked core mannose of the conserved mannosylchitobiose core of N-linked oligosaccharides.

    Recombinant from Xanthamonas manihotis in E. Coli


    View product documentation: Specsheet / CofA / MSDS

    • Part Number
    • E-AM02
    • Amount of Enzyme
    • 0.6 U / 60 µL






    Kit includes enzyme plus reaction buffer.

    Sufficient for up to 60 reactions.


  • α(1-3,4)-fucosidase


    Ludger Alpha-(1-3,4)-fucosidase enzyme structure

    E-F134

    α(1-3,4) Fucosidase cleaves branched non-reducing terminal fucose, linked α(1-3) or α(1-4) to the N-acetylglucosamine of terminal Gal-GlcNAc disaccharide structures.

    Xanthamonas manihotis


    View product documentation: Specsheet / CofA / MSDS

    • Part Number
    • E-F134
    • Amount of Enzyme
    • 30 mU / 60 µL






    Kit includes enzyme plus reaction buffer.

    Sufficient for up to 60 reactions.



Companion Products


Post-exoglycosidase glycan clean-up with EXO spin columns and 96 well plate
Check Ludger glycan clean up technology


Process controls for exoglycosidase digestions:
Range of labelled glycan standards

Ludger N-Glycan Nomenclature Table
Check N-Glycan nomenclature table


Glycan labelling kits
Ludger Glycan Labelling Summary Table
Check Ludger glycan labelling technology