Ludger Alpha-(1,6) core mannosidase enzyme structure

    α(1,6) Core Mannosidase


    E-AM02

    α(1-6) Core Mannosidase cleaves unbranched non-reducing terminal mannose, α(1-6) linked to the β-linked core mannose of the conserved mannosylchitobiose core of N-linked oligosaccharides.

     View product documentation: Specsheet / CofA / MSDS

    • Part Number
    • E-AM02
    • Amount of Enzyme
    • 0.6 U / 60 µL








    Kit includes enzyme plus reaction buffer.
    Sufficient for up to 60 reactions.






    Product Specifications:


    Core α Mannosidase cleaves unbranched non-reducing terminal mannose, α(1-6) linked to the β-linked core mannose of the conserved mannosylchitobiose core of N-linked oligosaccharides. The presence of fucose linked to the core N-acetylglucosamine has no effect on cleavage.

    The enzyme may inhibit other mannosidases, such as α(1-2,3,6) mannosidase (E-AM01), if a noncleavable α(1-6) mannose is present on the substrate. It should therefore always be added subsequent to digestion by other mannosidases.

    Applications:
    α(1-6) Core Mannosidase is useful for:
    Determining mannose linkages
    Rapidly removing the α(1-6) core mannose resistant to other mannosidases

    Source: recombinant from Xanthamonas manihotis E. Coli

    EC: 3.2.1.24

    Alternate Names: α-D-mannosidase, p-nitrophenyl-α-mannosidase, α-D-mannopyranosidase, core α mannosidase, exo-α-mannosidase

    Contents:
    Ludger alpha-(1,6) core mannosidase - Kit Contents
    Core Alpha Mannosidase in 50 mM sodium phosphate, 0.1 mM ZnCl (pH 7.5).
    200 µL 5x Reaction Buffer 5.0 (250 mM sodium phosphate, pH 5.0).

    Formulation:
    The enzyme is provided as a sterile solution in 50 mM sodium phosphate pH 7.5, 0.1 mM ZnCl2.

    Specific Activity: >1.5 U/mg
    Activity: 1 U/mL

    Molecular weight: ~52,000 daltons

    Suggested usage:
    1. Add up to 1 nmol of oligosaccharide.
    2. Add deionized water to 15 µL.
    3. Add 4 µL 5x Reaction Buffer 5.0.
    4. Add 1 µL of enzyme
    5. Incubate ten minutes at 37°C.

    Specific Activity Assay: One unit of α(1-6) Mannosidase is defined as the amount of enzyme required to produce 2 µmoles of mannose in 1 minute at 37°C, pH 5.0 from α(1-6) mannobiose.

    Storage: Store enzyme at 4°C.

    Purity: Each lot of α(1-6) mannosidase is tested for contaminating substances by incubating the enzyme for 24 hours at 37°C with substrates indicated in the table below. No detectable activity is evident for any of these potential contaminants. The detection limit of this assay is 5 µU/mL (IUB).

    The production host strain has been extensively tested and does not produce any detectable glycosidases.

    Stability: Stable at least 12 months when stored properly. Several days exposure to ambient temperatures will not reduce activity.





Companion Products:

Process controls - View our N-glycan Nomenclature Table


Glycan clean-up - removal of enzymes from glycan mixtures following exoglycosidase treatment

  • LC-EXO-A6
  • LC-EXO-96
  • LudgerClean Post-Exoglycosidase Clean-up Spin Columns (6 samples)
  • LudgerClean Post-Exoglycosidase Clean-up Plate (96 samples)

Labelling and derivatisation of released glycans - View our Glycan Labelling Summary Table

  • LT-KAB-A2
  • LT-KAB-VP24
  • LT-KAB-VP96
  • LT-KPROC-24
  • LT-KPROC-96
  • LT-KPROC-VP24
  • LT-KAA-A2
  • LT-KAA-VP24
  • LT-PERMET-96
  • LT-PERMET-VP96
  • LudgerTag 2-AB Glycan Labelling Kit, sodium cyanoborohydride (24 samples)
  • LudgerTag 2-AB Glycan Labelling Kit, picoline borane (24 samples)
  • LudgerTag 2-AB Glycan Labelling Kit, picoline borane (96 samples)
  • LudgerTag Procainamide Glycan Labelling Kit, sodium cyanoborohydride (24 samples)
  • LudgerTag Procainamide Glycan Labelling Kit, sodium cyanoborohydride (96 samples)
  • LudgerTag Procainamide Glycan Labelling Kit, picoline borane (24 samples)
  • LudgerTag 2-AA Glycan Labelling Kit, sodium cyanoborohydride (24 samples)
  • LudgerTag 2-AA Glycan Labelling Kit, picoline borane (24 samples)
  • LudgerTag Permethylation Kit (96 samples)
  • LudgerTag Permethylation Kit, without methyl iodide (96 samples)

Alternative enzymes

  • E-AM01
  • α(1-2,3,6)-mannosidase