Ludger Alpha-(1-2,3,6)-mannosidase enzyme structure

    α(1-2,3,6)-mannosidase


    E-AM01

    α Mannosidase from Jack Bean cleaves α(1-2,3,6)-linked mannose.

     View product documentation: Specsheet / CofA / MSDS

    • Part Number
    • E-AM01
    • Amount of Enzyme
    • 0.6 U / 60 µL








    Kit includes enzyme plus reaction buffer.
    Sufficient for up to 60 reactions.









    Product Specifications:


    α Mannosidase from Jack Bean cleaves α(1-2,3,6)-linked mannose.

    This enzyme is often used in conjunction with Core α(1-6) Mannosidase (E-AM02) if a noncleavable core α(1-6) mannose is present on the substrate.

    Source: Jack Bean

    EC: 3.2.1.24

    Alternate Names: α-D-mannosidase, p-nitrophenyl-α-mannosidase, α-D-mannopyranosidase, α mannosidase, exo-α-mannosidase

    Contents:
    Ludger alpha-(1-2,3,6)-mannosidase - Kit Contents
    α(1-2,3,6) Mannosidase in 150 mM sodium phosphate, 0.1 mM ZnCl2 pH 7.5. (pH 7.5)
    200 µL 5x Reaction Buffer 5.0 (250 mM sodium phosphate, pH 5.0)

    Specific Activity: >3 U/mg
    Activity: >10 U/mL

    Molecular weight: two polypeptides of 64,000 and 44,000 daltons

    Suggested usage:
    1. Add up to 1 nmol of oligosaccharide.
    2. Add deionized water to 15 µL.
    3. Add 4 µL 5x Reaction Buffer 5.0.
    4. Add 1 µL of enzyme
    5. Incubate ten minutes at 37°C.

    Specificity: All α(1-2,3,6)-linked mannose

    Specific Activity Assay:
    One unit of α Mannosidase is defined as the amount of enzyme required to hydrolyze 1 µmole of p-nitrophenyl-α-p-mannoside to p-nitrophenol in 1 minute at pH 5.0 and 37°C.

    Storage: Store enzyme at 4°C.

    Purity: Each lot of α(1-2,3,6) mannosidase is tested for contaminating substances by incubating the enzyme for 24 hours at 37°C with substrates indicated in the table below. No detectable activity is evident for any of these potential contaminants. The detection limit of this assay is 5 µU/mL (IUB).

    For the protease assay, 10 µg of denatured BSA is incubated for 24 hours with 2 µL of enzyme. Analysis of the BSA band after SDS-PAGE should show no evidence of degradation.

    Stability: Stable at least 12 months when stored properly. Several days exposure to ambient temperatures will not reduce activity.





Companion Products:

Process controls - View our N-glycan Nomenclature Table


Glycan clean-up - removal of enzymes from glycan mixtures following exoglycosidase treatment

  • LC-EXO-A6
  • LC-EXO-96
  • LudgerClean Post-Exoglycosidase Clean-up Spin Columns (6 samples)
  • LudgerClean Post-Exoglycosidase Clean-up Plate (96 samples)

Labelling and derivatisation of released glycans - View our Glycan Labelling Summary Table

  • LT-KAB-A2
  • LT-KAB-VP24
  • LT-KAB-VP96
  • LT-KPROC-24
  • LT-KPROC-96
  • LT-KPROC-VP24
  • LT-KAA-A2
  • LT-KAA-VP24
  • LT-PERMET-96
  • LT-PERMET-VP96
  • LudgerTag 2-AB Glycan Labelling Kit, sodium cyanoborohydride (24 samples)
  • LudgerTag 2-AB Glycan Labelling Kit, picoline borane (24 samples)
  • LudgerTag 2-AB Glycan Labelling Kit, picoline borane (96 samples)
  • LudgerTag Procainamide Glycan Labelling Kit, sodium cyanoborohydride (24 samples)
  • LudgerTag Procainamide Glycan Labelling Kit, sodium cyanoborohydride (96 samples)
  • LudgerTag Procainamide Glycan Labelling Kit, picoline borane (24 samples)
  • LudgerTag 2-AA Glycan Labelling Kit, sodium cyanoborohydride (24 samples)
  • LudgerTag 2-AA Glycan Labelling Kit, picoline borane (24 samples)
  • LudgerTag Permethylation Kit (96 samples)
  • LudgerTag Permethylation Kit, without methyl iodide (96 samples)

Alternative enzymes

  • E-AM02
  • α(1,6) core mannosidase






References:

1. Snaith, S.M., and G.A. Levvy. Purification and Properties of α-D- Mannosidase from Jack-Bean meal. Biochem. J. 110: 633-670 (1968)

2. Ganesh Kumar, B.S., Pohlrntz, G., Shculte, M., Mormann, M. Siva Kumar, N. Jack bean α-mannosidase: amino acid sequencing and N-glycosylation analysis of a valuable glycomics tool. Glycobiology 24(3):252-61 (2014)