- Part Number
- E-AM01
- Amount of Enzyme
- 0.6 U / 60 µL

α(1-2,3,6)-mannosidase
E-AM01
α Mannosidase from Jack Bean cleaves α(1-2,3,6)-linked mannose.
View product documentation: Specsheet / CofA / MSDS
Kit includes enzyme plus reaction buffer.
Sufficient for up to 60 reactions.
Product Specifications:
α Mannosidase from Jack Bean cleaves α(1-2,3,6)-linked mannose.
This enzyme is often used in conjunction with Core α(1-6) Mannosidase (E-AM02) if a noncleavable core α(1-6) mannose is present on the substrate.
Source: Jack Bean
EC: 3.2.1.24
Alternate Names: α-D-mannosidase, p-nitrophenyl-α-mannosidase, α-D-mannopyranosidase, α mannosidase, exo-α-mannosidase
Contents:
α(1-2,3,6) Mannosidase in 150 mM sodium phosphate, 0.1 mM ZnCl2 pH 7.5. (pH 7.5)
200 µL 5x Reaction Buffer 5.0 (250 mM sodium phosphate, pH 5.0)
Specific Activity: >3 U/mg
Activity: >10 U/mL
Molecular weight: two polypeptides of 64,000 and 44,000 daltons
Suggested usage:
1. Add up to 1 nmol of oligosaccharide.
2. Add deionized water to 15 µL.
3. Add 4 µL 5x Reaction Buffer 5.0.
4. Add 1 µL of enzyme
5. Incubate ten minutes at 37°C.
Specificity: All α(1-2,3,6)-linked mannose
Specific Activity Assay:
One unit of α Mannosidase is defined as the amount of enzyme required to hydrolyze 1 µmole of p-nitrophenyl-α-p-mannoside to p-nitrophenol in 1 minute at pH 5.0 and 37°C.
Storage: Store enzyme at 4°C.
Purity: Each lot of α(1-2,3,6) mannosidase is tested for contaminating substances by incubating the enzyme for 24 hours at 37°C with substrates indicated in the table below. No detectable activity is evident for any of these potential contaminants. The detection limit of this assay is 5 µU/mL (IUB).
For the protease assay, 10 µg of denatured BSA is incubated for 24 hours with 2 µL of enzyme. Analysis of the BSA band after SDS-PAGE should show no evidence of degradation.
Stability: Stable at least 12 months when stored properly. Several days exposure to ambient temperatures will not reduce activity.
Companion Products:
Process controls - View our N-glycan Nomenclature Table
- CN-MAN5-01
- CAB-MAN5-01
- CPROC-MAN5-01
- Man-5 glycan, unlabelled
- Man-5 glycan, 2-AB labelled
- Man-5 glycan, procainamide labelled
Glycan clean-up - removal of enzymes from glycan mixtures following exoglycosidase treatment
- LC-EXO-A6
- LC-EXO-96
- LudgerClean Post-Exoglycosidase Clean-up Spin Columns (6 samples)
- LudgerClean Post-Exoglycosidase Clean-up Plate (96 samples)
Labelling and derivatisation of released glycans - View our Glycan Labelling Summary Table
- LT-KAB-A2
- LT-KAB-VP24
- LT-KAB-VP96
- LT-KPROC-24
- LT-KPROC-96
- LT-KPROC-VP24
- LT-KAA-A2
- LT-KAA-VP24
- LT-PERMET-96
- LT-PERMET-VP96
- LudgerTag 2-AB Glycan Labelling Kit, sodium cyanoborohydride (24 samples)
- LudgerTag 2-AB Glycan Labelling Kit, picoline borane (24 samples)
- LudgerTag 2-AB Glycan Labelling Kit, picoline borane (96 samples)
- LudgerTag Procainamide Glycan Labelling Kit, sodium cyanoborohydride (24 samples)
- LudgerTag Procainamide Glycan Labelling Kit, sodium cyanoborohydride (96 samples)
- LudgerTag Procainamide Glycan Labelling Kit, picoline borane (24 samples)
- LudgerTag 2-AA Glycan Labelling Kit, sodium cyanoborohydride (24 samples)
- LudgerTag 2-AA Glycan Labelling Kit, picoline borane (24 samples)
- LudgerTag Permethylation Kit (96 samples)
- LudgerTag Permethylation Kit, without methyl iodide (96 samples)
Alternative enzymes
- E-AM02
- α(1,6) core mannosidase
References:
1. Snaith, S.M., and G.A. Levvy. Purification and Properties of α-D- Mannosidase from Jack-Bean meal. Biochem. J. 110: 633-670 (1968)
2. Ganesh Kumar, B.S., Pohlrntz, G., Shculte, M., Mormann, M. Siva Kumar, N. Jack bean α-mannosidase: amino acid sequencing and N-glycosylation analysis of a valuable glycomics tool. Glycobiology 24(3):252-61 (2014)