Ludger Beta-(1-3,4,6)-galactosidase enzyme structure

    β(1-3,4,6)-galactosidase


    E-BG02

    Cleaves all β(1-3) and β(1-4) linked non-reducing, terminal galactose. β(1-6) linked galactose is released at a slower rate.

     View product documentation: Specsheet / CofA / MSDS

    • Part Number
    • E-BG02
    • Amount of Enzyme
    • 0.5 U / 200 µL








    Kit includes enzyme plus reaction buffer.
    Sufficient for up to 200 reactions.






    Product Specifications:


    β(1-3,4,6) Galactosidase cleaves all β(1-3) and β(1-4) linked non-reducing, terminal galactose. β(1-6) linked galactose is released at a slower rate. The enzyme is a glycoprotein.

    β(1-4) galactose is by far the most common linkage found in N-linked oligosaccharides. For cleavage of this linkage we recommend β(1-4) Galactosidase (E-BG07).

    Source: Bovine Testes

    EC: 3.2.1.23

    Alternate Names: β-D-galactoside galactohydrolase, Exo-(1-4)-β-D-galactanase, Lactase, β(1-3,4,6) galactosidase

    Contents:
    Ludger Beta-(1-3,4,6)-galactosidase - Kit Contents
    200 µL of β(1-3,4,6) Galactosidase in 20 mM Tris-HCl, 50 mM NaCl, 0.5mg/mL BSA, pH 7.5
    5x Reaction Buffer- 500 mM sodium citrate/phosphate pH 4

    The supplied buffer concentrate provides the optimal pH for enzyme activity with the standard substrate. If glycosidase treatment is performed at suboptimal pH because of glycoprotein solubility or activity requirements, expect some diminution in enzyme activity.

    Specific Activity: >10.2 U/mg
    Activity: >2.5 U/mL

    Molecular weight: 68,000 daltons

    Rxn pH: 4

    Suggested usage:
    1. Add up to 100 µg of asialoglycoprotein or 1 nmol of oligosaccharide to tube.
    2. Add deionized water to 14 µL.
    3. Add 4 µL 5x Reaction Buffer 4.0.
    4. Add 2 µL of enzyme
    5. Incubate one hour at 37°C.

    For glycoproteins, cleavage may be monitored by SDS-PAGE if the size differential between native and de-galactosylated protein is sufficient for detection.

    Specificity: Cleaves all β(1-3) and β(1-4) linked non-reducing, terminal galactose.

    Specific Activity: One unit of β(1-3,4,6) Galactosidase is defined as the amount of enzyme required to produce 1 µmole of p-nitrophenol (pNP) in 1 minute at 37°C, pH 4.0 from p-nitrophenyl-β-D-galactopyranoside.

    Storage: Store enzyme at -20°C.

    Purity: Each lot of β(1-3,4,6) Galactosidase is tested for contaminating protease as follows: 10 µg of denatured BSA is incubated for 24 hours at 37°C with 2 µL of enzyme. SDS-PAGE analysis of the treated BSA shows no evidence of degradation.

    Each lot is also tested for contaminating activities by incubating the enzymes with the appropriate substrates for 24 hours; the detection limit is 5 µU/mL (IUB). A passing lot will have no detectable activity.

    Stability: Stable at least 12 months when stored frozen.





Companion Products:

Process controls - View our N-glycan Nomenclature Table


Glycan clean-up - removal of enzymes from glycan mixtures following exoglycosidase treatment

  • LC-EXO-A6
  • LC-EXO-96
  • LudgerClean Post-Exoglycosidase Clean-up Spin Columns (6 samples)
  • LudgerClean Post-Exoglycosidase Clean-up Plate (96 samples)

Labelling and derivatisation of released glycans - View our Glycan Labelling Summary Table

  • LT-KAB-A2
  • LT-KAB-VP24
  • LT-KAB-VP96
  • LT-KPROC-24
  • LT-KPROC-96
  • LT-KPROC-VP24
  • LT-KAA-A2
  • LT-KAA-VP24
  • LT-PERMET-96
  • LT-PERMET-VP96
  • LudgerTag 2-AB Glycan Labelling Kit, sodium cyanoborohydride (24 samples)
  • LudgerTag 2-AB Glycan Labelling Kit, picoline borane (24 samples)
  • LudgerTag 2-AB Glycan Labelling Kit, picoline borane (96 samples)
  • LudgerTag Procainamide Glycan Labelling Kit, sodium cyanoborohydride (24 samples)
  • LudgerTag Procainamide Glycan Labelling Kit, sodium cyanoborohydride (96 samples)
  • LudgerTag Procainamide Glycan Labelling Kit, picoline borane (24 samples)
  • LudgerTag 2-AA Glycan Labelling Kit, sodium cyanoborohydride (24 samples)
  • LudgerTag 2-AA Glycan Labelling Kit, picoline borane (24 samples)
  • LudgerTag Permethylation Kit (96 samples)
  • LudgerTag Permethylation Kit, without methyl iodide (96 samples)

Alternative enzymes

  • E-BG07
  • E-AG02
  • β(1-4)-galactosidase
  • α(1-3,6)-galactosidase






References:

1. Guile GR, Rudd PM, Wing DR, Prime SB, Dwek RA. A rapid high resolution high-performance liquid chromatographic method for separating glycan mixtures and analyzing oligosaccharide profiles. Anal Biochem. 1996; 240(2):210-26.

2. Jacob GS, Scudder P. Glycosidases in structural analysis. Methods Enzymol. 1994;230:280-99.

3. Distler JJ, Jourdian GW. The purification and properties of beta-galactosidase from bovine testes. J Biol Chem. 1973; 248(19):6772-80.