α(1-3,4)-fucosidase




E-F134

α(1-3,4) Fucosidase cleaves branched non-reducing terminal fucose, linked α(1-3) or α(1-4) to the N-acetylglucosamine of terminal Gal-GlcNAc disaccharide structures.

 View product documentation: Specsheet / CofA / MSDS

• Part Number
• E-F134
• Amount of Enzyme
• 30 mU / 60 µL










Kit includes enzyme plus reaction buffer.
Sufficient for up to 60 reactions.








---





Product Specifications:




α(1-3,4) Fucosidase cleaves branched non-reducing terminal fucose, linked α(1-3) or α(1-4) to the N-acetylglucosamine of terminal Gal-GlcNAc disaccharide structures. The presence of sialic acid (but not fucose) linked to the galactose will block cleavage.

α(1-3, 4) Fucosidase is useful for:
- Fucose linkage determination
- Deglycosylating glycoproteins with Lewis structures

Source: Isolated from Xanthamonas manihotis

EC: 3.2.1.51

Alternate Names: α-L-fucoside fucohydrolase, α-L-fucosidase, α-(1-3,4) fucosidase

Contents:

α(1-3,4)-Fucosidase in 20 mM Tris-HCl, 25 mM NaCl,(pH 7.5).
5x Reaction Buffer 5.0 (250 mM sodium phosphate, pH 5.0)

Specific Activity: >2 U/mg
Activity: 0.5 U/mL

Molecular weight: 40,000 daltons

Formulation: The enzyme is provided as a sterile-filtered solution in 20 mM Tris-HCl, 25 mM NaCl pH 7.5.

Suggested usage:
1. Add up to 1 nmole of oligosaccharide to a tube.
2. Add de-ionized water to a total of 15 µL.
3. Add 4 µL of 5x Reaction Buffer 5.0.
4. Add 1 µL of α(1-3,4)-Fucosidase.
5. Incubate for 1 hour at 37°C.

Specificity: Non-reducing terminal branched fucose when linked α(1-3) or α(1-4) to GlcNAc of a Gal-GlcNAc disaccharide structure. The presence of sialic acid (but not fucose) linked to the galactose will block cleavage.

Specific Activity Assay: One unit of Fucosidase activity is defined as the amount of enzyme required to cleave 1 µmole of fucose from Lewis X trisaccharide, 4-methylumbelliferyl glycoside in 1 minute at 37°C and pH 5.0. Lewis X trisaccharide is Gal β-(1-4)[Fuc α-(1-3)]GlcNAc.

Storage: Store enzyme at 4°C.

Purity: Each lot of α(1-3,4) Fucosidase is tested for contaminating activities by incubating the enzyme for 24 hours at 37°C with the appropriate substrates; the detection limit of this assay is 5 µU/mL (IUB). A passing lot will have no detectable activity.

For the protease assay, 10 µg of denatured BSA is incubated for 24 hours with 2 µL of enzyme. Analysis of the BSA band after SDS-PAGE should show no evidence of degradation.

Stability: Stable at least 12 months when stored properly. Several days exposure to ambient temperatures will not reduce activity.

---

Companion Products:

Glycan clean-up - removal of enzymes from glycan mixtures following exoglycosidase treatment

• LC-EXO-A6
• LC-EXO-96
• LudgerClean Post-Exoglycosidase Clean-up Spin Columns (6 samples)
• LudgerClean Post-Exoglycosidase Clean-up Plate (96 samples)

Labelling and derivatisation of released glycans - View our Glycan Labelling Summary Table

• LT-KAB-A2
• LT-KAB-VP24
• LT-KAB-VP96
• LT-KPROC-24
• LT-KPROC-96
• LT-KPROC-VP24
• LT-KAA-A2
• LT-KAA-VP24
• LT-PERMET-96
• LT-PERMET-VP96
• LudgerTag 2-AB Glycan Labelling Kit, sodium cyanoborohydride (24 samples)
• LudgerTag 2-AB Glycan Labelling Kit, picoline borane (24 samples)
• LudgerTag 2-AB Glycan Labelling Kit, picoline borane (96 samples)
• LudgerTag Procainamide Glycan Labelling Kit, sodium cyanoborohydride (24 samples)
• LudgerTag Procainamide Glycan Labelling Kit, sodium cyanoborohydride (96 samples)
• LudgerTag Procainamide Glycan Labelling Kit, picoline borane (24 samples)
• LudgerTag 2-AA Glycan Labelling Kit, sodium cyanoborohydride (24 samples)
• LudgerTag 2-AA Glycan Labelling Kit, picoline borane (24 samples)
• LudgerTag Permethylation Kit (96 samples)
• LudgerTag Permethylation Kit, without methyl iodide (96 samples)