Enzymes (Endo- and Exoglycosidases) including LudgerZyme™ (LZ) products


Endoglycosidases:

Enzymes to cleave glycans from glycoproteins

  • PNGase F (Peptide N Glycosidase F)

    E-PNG01

    PNGase F (QABio). Sufficient for up to 60 reactions.

    0.3 U / 60 μl. Kit includes enzyme plus reaction buffers.

    Enzyme Commission number: EC 3.5.1.52

  • E-PNG05

    PNGase F (QABio). Sufficient for up to 200 reactions.

    1 U / 200 μl. Includes enzyme only.

    Enzyme Commission number: EC 3.5.1.52

  • Recombinant PNGase F

    E-rPNG01

    Recombinant PNGase F (QABio). Sufficient for up to 60 reactions.

    0.3 U / 60 μl. Kit includes enzyme plus reaction buffers.

    Enzyme Commission number: EC 3.5.1.52

  • LZ-rPNGaseF-kit *new product*

    LudgerZyme recombinant PNGase F kit (New England BioLabs). Sufficient for up to 150 reactions.

    150 μl. Kit includes enzyme plus reaction buffers.

    Enzyme Commission number: EC 3.5.1.52

    Product guide

    Certificates of analysis:   Batch # B7CC-01

  • Endoglycosidase F1

    E-EF01

    1 U / 60 μl. Selective release of high mannose and some hybrid type N-glycans from peptides and proteins.

  • Endoglycosidase F2

    E-EF02

    0.3 U / 60 μl. Selective release of biantennary and high mannose (at a 40X reduced rate) N-glycans from peptides and proteins.

  • Endoglycosidase F3

    E-EF03

    0.33 U / 60 μl. Selective release of triantennary and fucosylated biantennary N-glycans from peptides and proteins.

  • Endoglycosidase H

    E-EHO2

    0.3 U / 60 μl. Release of asparagine-linked hybrid or high mannose oligosaccharides but not complex oligosaccharides.

  • O-glycosidase

    E-G001

    75 mU / 60 μl

  • Endo-Beta-Galactosidase

    E-XBG01

    0.9 U / 60 μl. Cleaves internal ß(1-4) galactose linkages in unbranched, repeating poly-N-acetyllactosamine structures.

  • LudgerZyme Ceramide Glycanase kit

    LZ-CER-HM-KIT

    Ceramide glycanase deglycosylates a variety of glycosphingolipids by cleaving the ß-glycosyl linkage.

    Enzyme Commission number: EC 3.2.1.123

    Product overview

    Product guide

    Certificate of stability

    Certificates of analysis:   Batch # B54O-02   Batch # B66S-02   Batch # B6B7-01   Batch # B7A3-01


Exoglycosidases:

Enzymes to cleave specific terminal monosaccharides from glycans

  • Alpha-(1-2,3,6)-mannosidase

    E-AM01

    0.6 U / 60 μl

  • Alpha-(1,6) core mannosidase

    E-AM02

    0.6 U / 60 μl

  • Alpha-(1-3,6)-galactosidase

    E-AG01

    30 U/mg / 400 U/ml

  • Beta-(1-3,4,6)-galactosidase

    E-BG02

    0.5 U / 200 μl

  • Beta-(1-4)-galactosidase

    E-BG07

    180 mU / 60 μl

  • Alpha-(1-3,4)-fucosidase

    E-F134

    30 mU / 60 μl

  • Beta-N-acetylglucosaminidase

    E-GL01

    2.4 U / 60 μl

  • Sialidase Au Alpha-(2-3,6,8,9)

    E-S001

    0.3 U / 60 μl

  • Sialidase Cp Alpha-(2-3,6)

    E-S005

    900 mU / 60 μl

  • Sialidase Sp Alpha-(2-3)

    E-S007

    300 mU / 60 μl

  • LudgerZyme Acetyl esterase (sialate-O-acetylesterase) kit

    LZ-ACASE-KIT

    To remove 9-, 8- and 7-O-acetyl groups from released sialic acids, released glycans or glycoproteins.
    Used for characterisation of highly sialylated biotherapeutics such as EPO, FSH and blood clotting factors.

    Enzyme Commission number: EC 3.1.1.53

    Product guide

    Certificate of stability

    Certificates of analysis:   Batch # B617-04