Ludger Procainamide Glycan Labeling

LudgerTag Procainamide Glycan Labeling Kit

Procainamide labeling permits glycan identification by either mass spectrometry or (U)HPLC, and because of its improved ionisation efficiency
compared to 2AB labeling it can permit identification of minor glycans (<1% relative peak area) by ESI-MS.

Ludger's procainamide labeling system is suitable for N-glycans, O-glycans, GSL-glycans, heparin or any sugar with a reducing terminus.

Our labeling kit uses the same reductive amination labeling method that has been used for 2AB & 2AA.
Two procainamide labeling kits are available; LT-KPROC-24 and LT-KPROC-VP24.

The LT-KPROC-24 kit contains sodium cyanoborohydride as a reductant. This is a strong reagent, producing labeled glycans in high yields.

The LT-KPROC-VP24 kit contains 2-picoline borane (2-PB) as a reductant. This reductant is less toxic than sodium cyanoborohydride. The yields of fully reduced labeled glycans might be lower when compared to sodium cyanoborohydride reductant.

Once labelled with procainamide the glycans are suitable for incubation with exoglycosidases.

We also offer a clean up system for use after labeling and a range of procainamide labeled glycan standards (including an N-glycan library and glucose homopolymer ladder).

The Table below outlines the features and benefits of Ludger's procainamide labeling technology in comparison to competitor offerings:

Ludger Procainamide Rapid/instant labelling*
Sensitive fluorescence detection for relative glycan quantitation by (U)HPLC ✓✓✓✓✓ ✓✓✓✓✓
Sensitive ESI-MS and MS/MS detection for glycan structural analysis ✓✓✓✓✓ ✓✓✓✓✓
Sample processing speed ✓✓✓ ✓✓✓✓✓
Can label purified glycans and standards? YES NO
Use for O-glycans? YES NO
Use for GSL glycans? YES NO
Similar glycan derivatisation chemistry to 2-AB industry standard? YES NO
Suitable for exoglycosidase sequencing? YES Fluorophore dependent**
* rapid/instant labelling technologies use N-hydroxysuccinimide (NHS) activated fluorophores that react with the glycosylamine form of the glycan
** aminoquinoline labels are not compatible with Bovine Kidney Fucosidase (BKF)


The presentation below relates to our LT-KPROC-VP24 kit. Results for LT-KPROC-24 are directly comparable.

LudgerTag Procainamide Glycan Labeling System

  Quick Links:

  1. Introduction: LudgerTag Procainamide Glycan Labeling System

  2. How we are using the LudgerTag Procainamide Labeling System

  3. Next Steps

  Product/Ordering Information:

Cat #

Posters & Papers

Analytical Chemistry Kozak

Analytical Biochemistry

2014 October;486:38-40. doi:10.1016/j.ab.2015.06.006. Epub 1 October 2015
Comparison of procainamide and 2-aminobenzamide labeling for profiling and identification of glycans by liquid chromatography with fluorescence detection coupled to electrospray ionization-mass spectrometry
Kozak R, Tortosa C, Fernandes D, Spencer D.

Ludger poster

Investigating the role of insect vector glycosylation in African sleeping sickness transmission: Characterisation of procainamide-labelled tsetse fly saliva N-glycans

Kozak RP, Wongtrakul-Kish K, Williams C, Fernandes DL, Perally S, Rose C, Spencer DI, Acosta-Serrano A
Presented at Glyco23: 23rd International Symposium on Glycoconjugates
Split, Croatia, September 2015

Ludger poster

Procainamide labelling as part of a flexible glycoprofiling system for monitoring of Gal-a1-3Gal related Glycosylation Critical Quality Attributes (GCQAs) of monoclonal antibody (mAb) therapeutics throughout the product life cycle

Kozak RP, Royle L, Liew LP, Spencer DI, Fernandes DL
Presented at WCBP 2016: 20th Symposium on the Interface of Regulatory and Analytical Sciences for Biotechnology Health Products
Washington DC, United States, January 2016

Ludger poster

A QbD-compatible approach for reliable measurement of sialic acid O-acetylation as a potential Glycosylation Critical Quality Attribute (GCQA) of erythropoietin (EPO) therapeutics

Fernandes DL, Thomson R, Gardner R, Liew LP, Kozak RP, Royle L, Strohfeldt K, Osborn H, Spencer DI
Presented at WCBP 2016: 20th Symposium on the Interface of Regulatory and Analytical Sciences for Biotechnology Health Products
Washington DC, United States, January 2016


Technical Questions

Radoslaw Kozak

Dr. Radoslaw Kozak
Senior Scientist

Quotations and Orders

Karen Oakes

Karen Oakes
Sales Manager