High Throughput Analysis of mAbs using GX-mAb

The presentation below outlines the service that Ludger has developed for high throughput glycan analysis of mAbs. This is suited to early stage drug developers who wish to compare glycosylation in a large number of samples. The service has comparatively short turnaround times and a choice of methods and analytical platforms. With GX-mAb you can get meaningful results on structural glycosylation allowing you to decide which are the best candidates to take through the drug development cycle.

High throughput glycan analysis service

  Presentation Chapters:

  1. Introduction

  2. Regulatory Landscape for mAb Glycosylation

  3. Orthogonal Glycoanalytical Platforms

  4. Robust, Validated Technology

  5. How does GX-mAb service work?

  Submitting samples to Ludger


  1. Ludger will send you a sample submission form in an automated excel format, and a 96-well sample plate. Click for a preview of the main form and sample form.
    Fill in the sample submission documents with information about your samples (sample ID and amount). You can put samples and their replicates in any of the unmarked positions on the plate layout in the sample submission form. We provide internal positive and negative control samples for processing through the sample preparation method but we recommend that you provide at least one negative control blank sample (e.g buffer). You may also provide your own internal standards.

  2. For optimal sample analysis, we require 100 µg of purified glycoprotein for each sample. Add your samples to the appropriate wells of the sample plate corresponding to the layout in the sample submission form.

  3. Dry down your samples in the plate before sending to Ludger (If the samples are sent as a solution in the plate there will be a small extra cost for us to dry down the samples prior to analysis).

  4. Seal the plate using either the foil seal or strips of caps that are provided.

  5. Send your samples to Ludger using the most appropriate conditions to keep your samples stable during their transit.


Can I send less of each sample?

100 µg of purified sample is the optimal amount for sample preparation and analysis. It is possible to perform the process using less sample but this would have an impact on processing time and, therefore, turnaround time.

Can I send the samples in solution?

Samples can be sent to us in solution distributed on the plate (please use a maximum volume of 150 µL per well). Depending on the stability of your samples in solution, the samples can be sent at room temperature, 4-8 °C or frozen. However, there will be a charge for us to dry down the samples at Ludger.

Alternatively, samples can be sent to us in individual vials. For this there will be an additional charge for transferring the samples to a plate and drying down the samples at Ludger ready for sample processing.

My samples are present in buffer following sample processing. Which buffers are compatible with the sample preparation methods?

For optimal sample preparation and analysis, please provide purified samples that have been dried down from a water or 0.1% TFA solution. Detergent, surfactants and other protein stabilisers e.g. PS80 can produce a gel when dried down and interfere with our sample preparation methods.

If you have to send samples to us in solution due to the instability of dried samples, please send them to us in water or a 0.1% TFA solution.

If you have to send samples to us in a buffer solution, detergents, surfactants and other protein stabilisers must be removed and buffer concentration should be kept to a minimum. Also note that, free saccharides in solution can also impact sample preparation methods and analysis. If you have any concerns about buffer content of your samples then contact us.

I don’t have a plate sealer, how can I seal the plate?

Please use the caps that are provided as part of the kit. When using the caps, make sure that the caps are pushed tightly into the wells to prevent any solution leaking.

Can I send other samples for you to analyse other than mAbs?

Yes, you can send other glycoprotein samples and we can analyse them using our custom glycoprofiling service.


Technical Questions

Paulina Urbanowicz

Paulina Urbanowicz
Senior Scientist


Dr. Richard Gardner

Dr. Richard Gardner
Lead Scientist