Mannose 6 phosphate quantitative standard
Mannose-6-Phosphate standard, 10nmol (CM-MAN6P-10), which is a quantified monosaccharide reference standard and has been validated using quantitative NMR. We recommend using this standard to assess the Man6P content while characterising your recombinant therapeutic glycoproteins.
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therapeutic glycoproteins contain negatively charged glycans such as
sialylated, sulphated and phosphorylated glycans that impact their
structure, function, safety, and efficacy. Therefore, it is a regulatory
requirement to analyse the Glycosylation Critical Quality Attributes (GCQA) of
Mannose-6-Phosphate (Man6P), a terminal monosaccharide of
asparagine-linked oligosaccharides is a key targeting signal for acid hydrolase
precursor proteins that are destined for transport to lysosomes and is present
in therapeutic enzymes (enzyme replacement therapies) developed for the treatment of lysosomal storage diseases.
This Mannose-6-Phosphate reference
standard (CM-MAN6P-10), which is a quantified monosaccharide has
been validated using quantitative NMR. We recommend using this standard to
assess the Man6P content while characterising your recombinant therapeutic
Form: Dry. Dried by centrifugal evaporation from an aqueous solution.
Amount: 10 nmol
Storage: -20˚C both before and after dissolution. This product is stable for at least 5 years as supplied.
Shipping: Shipped at ambient when dry. After dissolution, ship on dry ice.
Handling: Allow the unopened vial to reach ambient temperature and tap unopened on a solid surface to ensure that most of the lyophilized material is at the bottom of the vial. Gently remove the cap, add the desired volume of reconstitution medium, re-cap and mix thoroughly to bring all the oligosaccharides into the solution. For maximal recovery of oligosaccharide, ensure that the cap lining is also rinsed and centrifuge the reconstituted vial briefly before use. Ensure that any glass, plasticware or solvents used are free of glycosidases and environmental carbohydrates—Minimise exposure to elevated temperatures or extremes of pH. High temperatures and low pH will cause desialylation. High pH will cause epimerisation of the reducing terminus GlcNAc.
Safety: This product is non-hazardous and has been purified from natural sources certified to be free of all hazardous material including pathogenic biological agents.