LudgerClean protein-binding membrane plate for unlabelled glycan enrichment

LudgerClean protein-binding membrane plate for unlabelled glycan enrichment


  • Product Code: LC-PBM-96
  • Size 1 x 96 well plate

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Each pack contains one hydrophobic PVDF 96-well plate. For removal of enzymes and other proteins from unlabelled glycan mixtures.
For use in a vacuum manifold system (e.g. Ludger LV system) or centrifuge equipped with 96-well plate rotor.

Post-exoglycosidase clean-up:
For removal of exoglycosidase enzymes and other protein and peptide contaminants from unlabeled glycan mixtures. This prevents contamination of HPLC columns during subsequent chromatographic analysis. The plate can also be used to remove proteins before mass spectrometry analysis of glycans.

Post-endoglycosidase Clean-up:
For removal of endoglycosidase enzymes and other protein and peptide material following enzymatic release of glycans from glycoproteins. Using this protein binding membrane will provide cleaner samples and increase the signal for the subsequent fluorescent labelling of the glycans. The plate can also be used to remove the enzymes and other protein and peptide material before permethylation and/or mass spectrometry analysis of glycans.

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Product guide

Product Specifications

Applications:

Post-exoglycosidase clean-up
For removal of exoglycosidase enzymes and other protein and peptide contaminants from unlabeled glycan mixtures. This prevents contamination of HPLC columns during subsequent chromatographic analysis. The plate can also be used to remove proteins before mass spectrometry analysis of glycans.

Post-endoglycosidase Clean-up
For removal of endoglycosidase enzymes and other protein and peptide material following enzymatic release of glycans from glycoproteins. Using this protein binding membrane will provide cleaner samples and increase the signal for the subsequent fluorescent labelling of the glycans. The plate can also be used to remove the enzymes and other protein and peptide material before permethylation and/or mass spectrometry analysis of glycans.

Description: The LC-PBM-96 plate is a 96 well membrane-bottom plate containing a specialized protein binding membrane with a nominal pore size of 0.45 μm. This product is designed for use with both the vacuum manifold that can be purchased from Ludger and with a centrifuge equipped with a 96-well plate rotor. Glycans pass through the membrane whilst proteins are bound to the membrane, allowing separation of these two components.

Number of Samples: Sufficient for up to 96 samples.

Amount of Sample: Up to 350 μL per well

Suitable Samples: Underivatised glycans released enzymatically from glycoproteins including acidified N-glycans released from glycoproteins

Storage: Store at room temperature. Protect from sources of heat, light, and moisture. When stored correctly, the reagents should be stable for 36 months from date of purchase.

Shipping: The product should be shipped at ambient temperature.
For research use only. Not for human or drug use