LudgerClean A cartridges
Glycopeptide Enrichment: LudgerClean A Cartridges
To be able to enrich glycopeptides from a peptide/glycopeptide mixture after protease digestion.
Glycopeptides signals are attenuated in a complex mixture, as they are suppressed by non-glycosylated peptides. Performing a glycopeptide enrichment before analysis will enhance your UHPLC and/or MS signals. This clean-up/enrichment can be achieved by using the LudgerClean A cartridges which are supplied as a pack of 24 cartridges (Cat # LC-A-24). Enrichment with LC-A can be performed before or after labelling reaction.
LudgerClean A Cartridges
LudgerClean A cartridges are designed for use with any standard 96 well micro-titre plate compatible with a vacuum manifold (E.g. Ludger-Velocity Vacuum Manifold System - LC-VAC-MANIFOLD-KIT) or a liquid handling robot.
NOTE: The devices can be used without a vacuum manifold. In this case a pipette can be used to help push the sample through the cartridge by use of air displacement if gravity alone takes longer than 5 minutes. It is important to ensure that the contact times for the binding, washing and elution steps are consistent and gradual. Fast times may result in poor binding and selective elution. If a solution passing through a cartridge is slower than 5 minutes then use a higher vacuum pressure or pipette to speed up the process, allowing at least 1 minute solvent contact time with the cartridge.
Each pack contains 24 cartridges.
** LC-A cartridges can also be used for purification of glycans after fluorophore labelling.
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Quotations and Orders
Hydrophilic interaction amide cartridges. For purification of 2AA/or 2AB labelled N glycans, VTag labelled glycopeptides or APTS labelled N-glycans. Each pack contains 24 cartridges.
Can be used in a vacuum manifold system (e.g. Ludger LV system) that can process up to 96 cartridges simultaneously.
View product document:
Application: Glycopeptide enrichment following protease digestion can be achieved using LudgerClean A Cartridges, enhancing your UHPLC and/or MS signals. Glycopeptides signals are attenuated in a complex mixture, as they are suppressed by non-glycosylated peptides. Enrichment can be performed before or after a labeling reaction. The cartridges contain a unique solid phase extraction (SPE) resin that binds a wide range of glycopeptides and allows the enrichment of these from peptide/glycopeptide mixtures. They are compatible with many leading vacuum manifold SPE handling systems.
Description: For enrichment of glycopeptides after protease digestion (eg. Sequence grade trypsin, pronase). Suitable for enrichment/cleanup after glycopeptide labeling with AQC (6-aminoquinolyl-N-hydroxy-succinimidyl carbamate) and V-tag.
Binding Capacity: Each cartridge can typically bind glycopeptides released from up to 100 μg of glycoprotein.
Number of Samples: LudgerClean™ A cartridges are designed for single use only.
Suitable Samples: A wide range of glycopeptides can be purified. This includes the purification of glycopeptides that have been generated from different proteases, like trypsin or pronase.
Storage: Store at room temperature in the dark. Protect from sources of heat, light, and moisture. The cartridges are stable for at least two years as supplied.
Shipping: The product can be shipped at ambient temperature.
Handling: Ensure that any glass, plasticware or solvents used are free of glycosidases and environmental carbohydrates. Use powder-free gloves for all sample handling procedures and avoid contamination with environmental carbohydrate.
Safety: Please read the Material Safety Data Sheets (MSDS's) for all chemicals used. All processes involving hazardous reagents should be performed using appropriate personal safety protection - eyeglasses, chemically resistant gloves (e.g. nitrile), etc. - and where appropriate in a laboratory fume cupboard
For research use only. Not for human or drug use
Additional Reagents and Equipment Required
• Pure water (HPLC grade)
• Acetonitrile (HPLC grade)
• Trifluoroacetic Acid
• Manifold Cartridge Holder Plate (LP-HOLDER-96)
• Plug Pack, 12 strips of 8 plugs, to seal empty cartridges in preparation for vacuum (LP-PLUG-96)
• Deep-well collection plate (Collection Plate Pack, LP-COLLPLATE-2ML-96)
• Vacuum manifold