LudgerClean 96-well post-exoglycosidase clean-up plate

LudgerClean 96-well post-exoglycosidase clean-up plate


  • Product Code: LC-EXO-96
  • Size 1 x 96 well plate

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Each pack contains one 96-well clean-up plate. Optimised for removal of enzymes post-exoglycosidase digestion of glycans.
For use in a vacuum manifold system (e.g. Ludger LV system) or centrifuge equipped with 96-well plate rotor.

Post-exoglycosidase Clean-up: For removal of exoglycosidase enzymes and other protein material following glycan enzymatic digestion/ sequencing. This will prevent contamination of HPLC columns during subsequent chromatographic analysis. The plate can also be used to remove exoglycosidases or other proteins before mass spectrometry analysis of glycans.

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Product guide

Product Specifications


Applications:


The Ludger Exoglycosidase Clean-up Plate have been developed for the removal of enzymes and other protein material following glycan digestion or sequencing. This will prevent contamination of HPLC columns during subsequent chromatographic analysis. The 96-well plate can also be used to remove exoglycosidases or other proteins before mass spectrometry analysis of glycans.

Description: The LC-EXO-96 plate is a 96 well membrane-bottom plate containing a specialized modified polyethersulfone membrane with a molecular weight cut-off of approximately 30 kDa. This product is compatible with negative pressure systems (like most of popular vacuum manifold systems, including the model supplied by Ludger) or centrifuges equipped with 96-well format plate rotor. Glycans pass through the membrane whilst proteins are retained on the membrane allowing separation of these two components.

Number of Samples: Sufficient for up to 96 samples.

Amount of Sample: Up to 350 μL per well

Centrifugal Force: 1500 x g

Operating Vacuum: 10-20 in Hg (approx. 0.35-0.7 bar)

Suitable Samples:Unlabelled and fluorophore labelled (e.g. 2-AB, 2-AA or procainamide labelled) glycans released from glycoproteins or other sources and treated with exoglycosidase enzymes.

Storage: Store at room temperature. Protect from sources of heat, light, and moisture. When stored correctly, the products should be stable for 36 months from date of purchase.

Shipping: The product should be shipped at ambient temperature.
For research use only. Not for human or drug use