Monosacharide Analysis

Monosaccharide Analysis

Monosaccharide analysis is a regulatory requirement laid out in the ICH Q6B guidelines for characterisation of biopharmaceuticals. This information can be used at all stages of drug development as a method of determining the type of glycosylation (N-linked and/or O-linked) and the extent to which glycosylation has occurred. It can also be used to demonstrate consistency between batches for QC lot release during the manufacturing process.

Ludger’s monosaccharide analysis kit is easy to use, reliable and can be used for quantitative routine monosaccharide analysis. (Kit sufficient for 96 analyses).

  • Release neutral and amino monosaccharides from your glycoprotein samples by mild acid hydrolysis
  • Fluorescent labelling of released monosaccharides with 2-aminobenzoic acid (2AA)
  • Relative quantitative analysis of 2AA-labelled monosaccharides compared to standards (GlcN, GalN, Gal, Man, Glc, Fuc, Xyl) by (U)HPLC analysis
Monosaccharide release & labelling kit

Monosaccharide release & labelling kit

LT-MONO-96

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For release and labelling of neutral and amino monosaccharides from glycoprotein therapeutics and pre-released glycans. This kit can be used for full quantitative or routine monosaccharide analysis. Sufficient for 96 samples. The LudgerTag Monosaccharide Analysis Kit contains reagents for the release and fluorescent labeling of monosaccharides found on glycoprotein biopharmaceuticals and standards.

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