NGA4 glycan (A4), procainamide labelled
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Tetraantennary N-glycan that contains terminal N-acetylglucosamine residues, 20pmol.
NGA3 Glycan Synonyms: NGA4 N-linked oligosaccharide
LudgerTag procainamide labeled glycans can be analyzed by UHPLC, HPLC, ESI-MS, and LC-ESI-MS methods. Because of its improved ionization efficiency compared to 2-AB labeling, it can permit the identification of minor glycans (<1% relative peak area) by ESI-MS.
Description: Asialo-, agalacto-, tetra-antennary complex-type N-glycan (oligosaccharide).
Sources: NGA4 is a substructure of glycans found on several mammalian glycoproteins including human alpha-1 acid glycoprotein (hAAGP). This product is typically purified from the oligosaccharide pool released from hAAGP by hydrazinolysis using a combination of HPLC and glycosidase digestion.
Form: Dry. Dried by centrifugal evaporation from an aqueous solution.
Amount: 20 pmols
Purity: >90% pure as assessed by a combination of 1 H-NMR and HPLC.
Storage: -20˚C both before and after dissolution. This product is stable for at least 5 years as supplied.
Shipping: The product can be shipped at ambient when dry. After dissolution, ship on dry ice.
Handling: Allow the unopened vial to reach ambient temperature and tap unopened on a solid surface to ensure that most of the lyophilized material is at the bottom of the vial. Gently remove the cap, add the desired volume of reconstitution medium, re-cap and mix thoroughly to bring all the oligosaccharide into solution. For maximal recovery of oligosaccharide, ensure that the cap lining is also rinsed and centrifuge the reconstituted vial briefly before use. Ensure that any glass, plasticware or solvents used are free of glycosidases and environmental carbohydrates. Minimise exposure to elevated temperatures or extremes of pH. High temperatures and low pH will cause desialylation. High pH will cause epimerisation of the reducing terminus GlcNAc.
Safety: This product is non-hazardous and has been purified from natural sources certified to be free of all hazardous material including pathogenic biological agents.
HPLC Analysis of the NGA2 Procainamide Glycan
Fluorescence response Procainamide glycans show a much higher sensitivity as compared to 2-AB labeled glycans, providing peak heights three times higher than traditional 2-AB labeled glycans. The procainamide labeled sugars show comparable separation to HILIC-UHPLC assays with 2-AB.
Mass Spectrometry and Electrophoresis
Mass spectrometry response Due to its high ionization efficiency, procainamide shows much higher sensitivities the seen with 2-AB. When analyzed using ESI-MS and ESI-MS/MS, procainamide produces twenty-two times higher peaks than 2-AB. This provides a great platform for the detection and identification on minor peaks by MS/MS analysis..