LudgerSep uR2 UHPLC column – sialic acid

LudgerSep uR2 UHPLC column – sialic acid


  • Product Code: LS-UR2-2.1x100
  • Size 2.1 x 100 mm

  • £981
  • Ex Tax: £981

Analysis of sialic acids labeled with 1,2-diamino-4,5 methylenedioxybenzene.2HCl (DMB) using UHPLC.

The LudgerSep™ uR2 UHPLC column contains particles with an endcapped octadecylsilane coating optimized for hydrophobic chromatography

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Product guide

 SDS

Product specification


Application: Analysis of sialic acids labeled with 1,2-diamino-4,5 methylenedioxybenzene.2HCl (DMB) using UHPLC.

Description: The LudgerSep™ uR2 UHPLC column contains particles with an endcapped octadecylsilane coating optimized for hydrophobic chromatography.

Particles: 1.9 μm silica derivatized with octadecylsiliane coating. 175 Angstrom pore size.

Column size:

Cat # Diameter x Length Column Volume

LS-UR2-2.1x100

2.1 x 100 mm

0.34 ml

Column Tube: Stainless steel

Flow Rates: Typical flow rates = 0.1 – 0.6 mL/min.

Column Pressure: Pressure should not exceed 15000psi.

pH Range: 1- 11

Temperature: Typical operating temperature, 30°C, but increasing the temperature may improve resolution for some samples.
Temperature range = 15 - 60°C. 

Solvents: A typical solvent system for DMB labelled sialic acid analysis uses 7:9:84 methanol:acetonitrile:water as solvent A, and acetonitrile as solvent B. Solvents should be degassed. Avoid strong oxidants and anionic detergents.

Column Protection: It is good practice to install a 0.2 μm in-line filter in front of the column.

Suitable Sample: DMB labeled sialic acids.

Sample Preparation: Filter samples, if required, through a 0.2 μm filter. Avoid exposure of DMB labelled samples to light.

Handling: Ensure that any glass, plasticware or solvents used are free of glycosidases and environmental carbohydrates. Use powder-free gloves for all sample handling procedures and avoid contamination with environmental carbohydrate.

Safety:Please read the Safety Data Sheets (SDS's) for all chemicals used.
All processes involving labelling reagents should be performed using appropriate personal safety protection - eyeglasses, chemically resistant gloves (e.g. nitrile), etc. - and where appropriate in a laboratory fume cupboard

For research use only. Not for human or drug use

UHPLC System Requirements


The LudgerSep™ uR2 column is designed for use with the latest generation of UHPLC instruments capable of withstanding high flow pressures and fast sample analyses. In order to take advantage of the high resolving power of sub 3 μm particle size containing columns, we recommend keeping sample injection volumes at or below 5 μL and minimising system void volumes. Ideally use full loop injection. Tubing should be narrow bore (about 0.13 mm diameter or less) and detector flow cell volumes should be 10 μl or less. Although an example chromatogram is shown in this guide, retention times will vary dependent on the UHPLC system used.