LudgerSep N buffer x40 concentrate

LudgerSep N buffer x40 concentrate

  • Product Code: LS-N-BUFFX40
  • Size 50 ml, makes 2 litres buffer

  • £168

50ml of x40 LS-N buffer concentrate (2.0M ammonium formate buffer, pH 4.4).
This can be diluted with water to prepare LS-N buffer (50 mM ammonium formate buffer, pH 4.4) used in amide or HILIC (hydrophilic interaction liquid chromatography) HPLC analysis of LudgerTag fluorophore and UV-chromophore labelled glycans.

Product specification

Application: For preparation of LS-N buffer (50 mM ammonium formate buffer, pH 4.4) used in amide or HILIC (hydrophilic interaction liquid chromatography) HPLC analysis of LudgerTag™ fluorophore and UV-chromophore labeled glycans.

Description: 50ml of x40 LS-N buffer (2.0M ammonium formate buffer/solution) in a square HDPE bottle with leak-proof cap.

Usage: Dilute with de-gassed HPLC grade water (use 1 part of x40 buffer to 39 parts of water) to make LS-N buffer (50 mM ammonium formate, pH 4.4). The 50 ml of x40 buffer will make 2 litres of LS-N buffer. Use the LS-N buffer for glycan analysis with any of the following HPLC columns:

  • LudgerSep™ N series columns
  • Tosoh Amide-80 columns
  • Waters ACQUITY UPLC BEH glycan columns
  • GlycoSep N and N-Plus columns
  • Other HILIC HPLC columns for glycan analysis

Storage: Store unopened bottle below 25 oC. As with any HPLC solvent we recommend preparation of the solvent immediately before use. Take care that the undiluted/diluted
solvent is not exposed to excessive heat or sunlight as it contains volatile components. Stability times of the prepared solvent will vary according to your laboratory conditions. Longterm storage of the prepared solvent, ie longer than 1 month, at room temperature, may result in evaporation of some of the volatile components in the solvent leading to a change in retention times.

Additional Reagents and Equipment Required

Pure water: resistivity 18 MΩ-cm, particle free (>0.22 μm), TOC <10 ppb

  • Volumetric flask
  • De-gassing station for HPLC solvents*

* Optional - depending on your HPLC system

Safety and Handling

  • Please read the Material Safety Data Sheet (MSDS) and instruction protocol given in this guide before using this product.
  • All processes involving this reagent should be performed using appropriate personal safety protection - eyeglasses, good quality chemically resistant gloves (e.g. nitrile), etc. - and where appropriate in a laboratory fume cupboard.
  • Ensure that any glass, plasticware or solvents used are free of glycosidases and environmental carbohydrates. Use powder-free gloves for all sample handling procedures and avoid contamination with environmental carbohydrate.