Fetuin glycoprotein standard

Fetuin glycoprotein standard


  • Product Code: GCP-FET-250U
  • Size 250 µg

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The Fetuin Glycoprotein Standard was developed for use as a positive control during glycan release and labeling (particularly for DMB sialic acid analysis). Fetuin exists in a variety of glycoforms containing bi-, tri-, and tetraantennary N-linked oligosaccharides with variable sialylation, as well as O-linked glycans.

1 vial containing 250 μg fetuin glycoprotein

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SDS

Product Specification


The fetuin glycoprotein standard was developed for use during glycan release and labeling(particularly for DMB sialic acid analysis). Fetuin exists in a variety of glycoforms containing bi-, tri-, and tetra-antennary oligosaccharides with variable sialylation, as well as O-linked glycans.

Amount of Glycoprotein Supplied
GCP-FET-05         500 µg
GCP-FET-250U     250 µg
GCP-FET-50Ux4     50 µg per vial – 4 vials

Source Fetal calf serum

Form Dry. Lyophilised powder.

Molecular Weight 36 kDa (protein weight only)

Amount of Protein
GCP-FET-05         300 µg
GCP-FET-250U     150 µg
GCP-FET-50Ux4     32 µg per vial – 4 vials
In comparison to BSA standard, determined by BCA assay. Value rounded to nearest μg.

Analysis
Fetuin glycans were released from the Fetuin Glycoprotein Standard (Cat# GCP-FET-05) using PNGase-F.

Following release the glycans were labeled using 2-Aminobenzamide (2-AB) using the LudgerTag 2-AB Glycan Labeling Kit (Cat# LT-KAB-A2).

Figure 1 shows a LudgerSep N2 HPLC profile of bovine fetuin N-glycans. To thoroughly investigate the N-glycans we first separate them based on charge on a LudgerSep C3 column (Figure 2) and then run each fraction on a LudgerSep N2 column.

Storage Refrigerate (-20°C) both before and after dissolving. This product is stable for at least 5 years as supplied.

Shipping The product is shipped at ambient temperature.

Handling Once dissolved avoid repeated thawing and refreezing, storage over 3 hours at room temperature or above, exposure to light and long term exposure to acid as these will cause glycan desialylation.

Safety This product is non-hazardous and has been purified from natural sources certified to be free of all hazardous material including pathogenic biological agents.