A2 glycan (A2G2S2, G2S2), APTS labelled

A2 glycan (A2G2S2, G2S2), APTS labelled

  • Product Code: CAPTS-A2-01
  • Size 20 pmol

  • £64


Bunz SC, Rapp E, Neusüss C. Capillary electrophoresis/mass spectrometry of APTS-labeled glycans for the identification of unknown glycan species in capillary electrophoresis/laser-induced fluorescence systems. Anal Chem. 2013 Nov 5;85(21):10218-24. doi: 10.1021/ac401930j. Epub 2013 Oct 15.

Bunz SC, Cutillo F, Neusüß C. Analysis of native and APTS-labeled N-glycans by capillary electrophoresis/time-of-flight mass spectrometry. Anal Bioanal Chem. 2013 Oct;405(25):8277-84. doi: 10.1007/s00216-013-7231-4. Epub 2013 Aug 3.

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Wang CY, Hsieh YZ. Analysis of chitin oligosaccharides by capillary electrophoresis with laser-induced fluorescence. J Chromatogr A (2002) 979:431-438

Chen FT, Evangelista RA. Profiling glycoprotein n-linked oligosaccharide by capillary electrophoresis. Electrophoresis (1998) 19:2639-2644.

Guttman A, Chen FT, Evangelista RA, Cooke N.References High-resolution capillary gel electrophoresis of reducing oligosaccharides labeled with 1-aminopyrene-3,6,8-trisulfonate. Anal Biochem (1996) 233:234-242

Guttman A, Chen FT, Evangelista RA. Separation of 1-aminopyrene-3,6,8-trisulfonate-labeled asparagine-linked fetuin glycans by capillary gel electrophoresis. Electrophoresis (1996) 17:412-417.

APTS labelled disialylated biantennary oligosaccharide

Biantennary N-glycan that contains terminal sialic acid residues, 20pmol.
m/z: 2663.7477

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Product Specification

A2 APTS Glycan Synonyms: A2 N-linked oligosaccharide. G2S2, A2G2S2, A2G(4)2S(6)2

Description: Di-sialylated, bi-antennary complex-type N-glycan (oligosaccharide).

Sources: A2 glycan is found on many mammalian glycoproteins including serum transferrin, and fibrin. This product is typically purified from the oligosaccharide pool released from fibrin by hydrazinolysis using a combination of HPLC and glycosidase digestion.

Form: Dry. Dried by centrifugal evaporation from an aqueous solution. Contains ammonium salt to stabilise against desialylation.

Molecular Weight: 2664

Fluorescence: λex = 425 nm, λem = 503 nm.

Amount: 20 pmols

Purity: >80% pure as assessed by HPLC.

Storage: -20˚C both before and after dissolution. This product is stable for at least 5 years as supplied.

Shipping: The A2 APTS glycan can be shipped at ambient when dry. After dissolution, ship on dry ice.

Handling: Allow the unopened vial to reach ambient temperature and tap unopened on a solid surface to ensure that most of the lyophilized material is at the bottom of the vial. Gently remove the cap, add the desired volume of reconstitution medium, re-cap and mix thoroughly to bring all the oligosaccharide into solution. For maximal recovery of oligosaccharide, ensure that the cap lining is also rinsed and centrifuge the reconstituted vial briefly before use. Ensure that any glass, plasticware or solvents used are free of glycosidases and environmental carbohydrates. Minimise exposure to elevated temperatures or extremes of pH. High temperatures and low pH will cause desialylation. High pH will cause epimerisation of the reducing terminus GlcNAc.

Safety: This product is non-hazardous and has been purified from natural sources certified to be free of all hazardous material including pathogenic biological agents.