Ludger Velocity Labelling Kit

LudgerTag™ 2AB and 2AA Glycan Labelling Kits with Safer Reductant Fluorescent

Fluorescent labelling methods for glycoprofiling of therapeutic glycoproteins are well established and aid subsequent separation and quantitation using a range of techniques. These include high performance liquid chromatography (HPLC and UHPLC), capillary electrophoresis (CE), and mass spectrometry.

Of the fluorescent labels available, 2-aminobenzamide (2-AB) and 2-aminobenzoic acid (2-AA) are most widely used for oligosaccharide profiling. Existing 2-AA and 2-AB labelling kits use sodium cyanoborohydride as a reducing agent during glycan labelling. This reagent is toxic so a fume cupboard should be used during handling. To conform with emerging Health and Safety regulations we are now replacing these with our new VP glycan kits that use picoline borane which is a significantly safer reductant. This technology, developed in collaboration with Leiden University Medical Centre, The Netherlands, has now been patented

The VP kits have the same glycan labelling performance as the traditional kits but are safer, more convenient (fewer vials to mix) and the reagents are more stable.