Posters

Improved and Semi-Automated Reductive β-Elimination Workflow for Higher Throughput Protein O-glycosylation Analysis

Kotsias M, Kozak RP, Gardner RA, Wuhrer M, Spencer D
Presented at: Society for GlycoBiology 2018 Annual Meeting
New Orleans, USA. November 5-8th 2018

Glycan Standards as Key Tools for Robust and Reliable Analysis of Glycoproteins

Hendel J, Peel S, Spencer D
Presented at: 29th International Carbohydrate Symposium
Lisbon, Portugal. July 15-19th 2018

Awarded the Chemical Biology Poster Prize by the Royal Society of Chemistry's 'Organic & Biomolecular Chemistry' Journal

Biopharmaceutical follicle-stimulating hormone (FSH) characterisation: monitoring Glycosylation Critical Quality Attributes (GCQAs) using a procainamide labelling system for structural glycan analysis and LC-ESI-QTOF for glycopeptide mapping

Thomson RI, Gardner RA, Kozak RP, Smith J, Strohfeldt K, Osborn HMI, Spencer DI
Presented at: WCBP 2018: 22nd Symposium on the Interface of Regulatory and Analytical Sciences for Biotechnology Health Products
Washington D.C., United States. January 30-February 1st 2018

Sialylation: A Critical Glycosylation Quality Attribute for Biopharmaceuticals

Hendel JL, Kozak RP, Morgan CL, Royle L
Presented at: WCBP 2018: 22nd Symposium on the Interface of Regulatory and Analytical Sciences for Biotechnology Health Products
Washington D.C., United States. January 30-February 1st 2018

Development of a High Throughput Method for
O-Glycosylation Analysis

Kotsias M, Gardner R, Kozak RP, Wuhrer M, Spencer D
Presented at: 12th Jenner Glycobiology and Medicine Symposium: Translational Glycobiology
Dubrovnik, Croatia. May 6-9th 2017

Keywords: Colorectal cancer (CRC), O-glycosylation, permethylation, MALDI-TOF-MS, high throughput analysis, GlycoCan

Analysis of Sialic Acids in Biopharmaceuticals

Hendel J, Royle L, Kozak RP, Fernandes DL
Presented at: WCBP 2017: 21st Symposium on the Interface of Regulatory and Analytical Sciences for Biotechnology Health Products
Washington D.C., United States. January 24-26th 2017

Keywords: RP-HPLC, EPO, Mabs, fetuin, sialic acid, critical quality attribute (CQA), quantitation, Neu5Ac, Neu5Gc, DMB

Automated High-Throughput Permethylation for Glycosylation Analysis Using MALDI-TOF-MS

Shubhakar A, Spencer D, Wuhrer M, Fernandes DL
Presented at the 38th Annual Congress of Pharmacology Society of Chile
Castro, Chile. November 26-29th 2016

Keywords: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS), permethylation, high throughput (HTP), N-glycans, O-glycans

Procainamide labelling as part of a sensitive workflow for the identification and quantitation of potential glycan drug targets in parasite cell membrane, insect vector saliva and host tissue models of infection by HILIC-HPLC-ESI-MS/MS

Wongtrakul-Kish K, Kozak RP, Spencer D
Presented at the GlycoPar Symposium & Workshop
Liverpool, UK. September 12-14th 2016

Keywords: Procainamide, LC-MS, HILIC-HPLC-ESI-MS/MS, parasitic glycans, insect vector glycans, host glycans, GlycoPar

First steps in the development of a high throughput method for O-glycosylation analysis

Kotsias M, Kozak RP, Shubhakar A, Fernandes DL, Spencer D
Presented at the GlycoPar Symposium & Workshop
Liverpool, UK. September 12-14th 2016

Keywords: Colorectal cancer (CRC), O glycans, permethylation, MALDI-TOF-MS, GlycoCan

Biopharmaceutical Erythropoietin Characterisation: critical quality attribute (CQA) mapping using LC-ESI-Qtof and automated database searching of glycopeptide analytes

Spencer D, Smith J, Gardner R, Galvin B, Resemann A, Hufnagel P, Fernandes D
Presented at the 64th ASMS Conference:
American Society for Mass Spectrometry
San Antonio TX, United States. June 5-9th 2016

Keywords: erythropoietin (EPO), glycan, glycopeptide, LC-ESI-QTof-MS, ProteinScape, critical quality attribute (CQA)

Procainamide labelling as part of a flexible glycoprofiling system for monitoring of Gal-a1-3Gal related Glycosylation Critical Quality Attributes (GCQAs) of monoclonal antibody (mAb) therapeutics throughout the product life cycle

Kozak RP, Royle L, Liew LP, Spencer DI, Fernandes DL
Presented at WCBP 2016: 20th Symposium on the Interface of Regulatory and Analytical Sciences for Biotechnology Health Products
Washington DC, United States. January 2016

Keywords: Gal alpha 1-3 gal, IgG, monoclonal antibody (mAb), glycosylation critical quality attribute (GCQA), QbD, procainamide, UHPLC, ESI-MS/MS

A QbD-compatible approach for reliable measurement of sialic acid O-acetylation as a potential Glycosylation Critical Quality Attribute (GCQA) of erythropoietin (EPO) therapeutics

Fernandes DL, Thomson R, Gardner R, Liew LP, Kozak RP, Royle L, Strohfeldt K, Osborn H, Spencer DI
Presented at WCBP 2016: 20th Symposium on the Interface of Regulatory and Analytical Sciences for Biotechnology Health Products
Washington DC, United States. January 2016

Keywords: QbD, erythropoietin (EPO), sialate O-acetyl esterase (Nan S), glycosylation critical quality attribute (GCQA), sialic acids, DMB, procainamide, UHPLC, LC-MS

Investigating the role of insect vector glycosylation in African sleeping sickness transmission: Characterisation of procainamide-labelled tsetse fly saliva N-glycans

Kozak RP, Wongtrakul-Kish K, Williams C, Fernandes DL, Perally S, Rose C, Spencer DI, Acosta-Serrano A
Presented at Glyco23: 23rd International Symposium on Glycoconjugates
Split, Croatia. September 2015

Keywords: trypanosomiasis, saliva, 2AB, procainamide, UHPLC, ESI-MS/MS

A Fluorescent Labeling and Enrichment System for Glycopeptides Generated from Proteolytic Digestion of IgG mAbs;
A System That Can Be Used as Part of the Peptide Mapping Workflow

Hendel JL, Badia-Tortosa C, Spencer DI, Fernandes DL
Presented at USP: Glycosylation Analysis for Biopharmaceuticals Workshop
Washington DC, United States. August 2015

Keywords: glycopeptide, IgG, monoclonal antibody (mAb), peptide mapping, V-Tag, UHPLC, MALDI-MS

Reliability of a semi-automated, high throughput (HT) MS based glycomics system for discovery of glycan biomarkers and Quality by Design (QbD) studies of glycoprotein therapeutics

Shubhakar A, Spencer DI, Wuhrer M, Fernandes DL
Presented at Gordon Research Conference
Lucca, Italy. March 2015

Investigating the role of insect vector glycosylation in African sleeping sickness transmission: Characterisation of procainamide-labelled tsetse fly saliva N-glycans

Kozak RP, Wongtrakul-Kish K, Williams C, Perally S, Rose C, Spencer DI, Acosta-Serrano A
Presented at:
Gordon Research Conference
Lucca, Italy. March 2015
and CASSS Analytical Technologies Conference
Berlin, Germany. March 2015

Drug Glycosylation Analysis using Automated,
High Throughput, Orthogonal Methods

Shubhakar A, Kozak RP, Badia-Tortosa C, Gardner RA, Royle L, Wuhrer M, Spencer DI, Fernandes DL
Presented at PEGS 2014: Glycosylation Analysis for Biopharmaceuticals Workshop
Boston, United States. May 2014

Keywords: high throughput, permethylation, V-Tag, glycosylation critical quality attribute (GCQA), IgG, erythropeitin (EPO), UHPLC, MALDI-MS

Studies on Salivary Glycosylation

Urbanowicz PA, Kozak RP, Fernandes DL
Presented at ACTA: 10th European Symposium on Saliva
Egmond aan Zee, Netherlands. May 2014

Keywords: saliva, O glycans, Orela, 2AB, glucose homopolymer standard, T1 cartridges, UHPLC, MALDI-MS

Optimisation of drug glycosylation supported by an automated high throughput permethylation MALDI-MS method

Shubhakar A, Spencer DI, Kozak RP, Velez-Suberbie L, Bracewell DG, Fernandes DL, Wuhrer M
Presented at ICS: 27th International Carbohydrate Symposium
Bangalore, India. January 2014

Keywords: high throughput, glycosylation critical quality attribute (GCQA), IgG4, bioreactor, permethylation, UHPLC, MALDI-MS

Systematic Analysis of Drug Glycosylation Critical Quality Attributes (GCQAs)

Royle L, Kozak RP, Liew LP, Emery RJ, Fernandes DL
Presented at Glyco22: 22nd International Symposium on Glycoconjugates
Dalian, China. June 2013

Suppression of Peeling during the Release of O-glycans by Hydrazinolysis

Kozak RP, Royle L, Gardner RA, Fernandes DL, Wuhrer M
Presented at the 4th Warren Workshop
Athens, Georgia, United States. August 2012