Alpha-Gal standard (2-AB labelled)
Platts-Mills TA, Schuyler AJ, Tripathi A, Commins SP. Anaphylaxis to the carbohydrate side chain alpha-gal. Immunol Allergy Clin North Am 35:247-260. (2015)
Tharmalingam T, Adamczyk B, Doherty MA, Royle L, Rudd PM. Strategies for the profiling, characterisation and detailed structural analysis of N-linked oligosaccharides. Glycoconj J. 30:137-146 (2013)
Kozak RP, Tortosa CB, Fernandes DL, Spencer DI. Comparison of procainamide and 2-aminobenzamide labeling for profiling and identification of glycans by liquid chromatography with fluorescence detection coupled to electrospray ionization-mass spectrometry. Anal Biochem 486:38-40 (2015)
The 2-AB Alpha Gal glycan is a linear trisaccharide: Gal-alpha(1-3)-Gal-beta(1-4)-GlcNAc.
Truncated N-Glycan containing alpha-gal epitope, 100pmol.
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The 2-AB Alpha Gal glycan is a linear trisaccharide with sequence linkages Gal-alpha(1-3)-Gal-beta(1-4)-GlcNAc. This standard was developed as a positive control when using alpha galactosidase for biopharmaceutical glycoprofiling assays.
A 2-AA labeled Alpha Gal standard is also available.
Galactose-alpha-1,3-galactose, commonly known as alpha gal, is a carbohydrate found in mammalian meat products. Approximately 1% of immunoglobulins in humans are specific to alpha-gal, and these antibodies (anti- Gal) cause an allergic reaction. Due to its potential immunogenicity, it is important therefore to screen biopharmaceuticals for alpha-gal. Some drug developers are also investigating the use of anti-Gal for tumour therapies.
Form Dry. Dried by centrifugal evaporation from an aqueous solution.
Number of Samples Typically, 100 fmol to 10 pmol of the Alpha Gal 2-AB glycan can be detected, dependent on the sensitivity of the fluorometer, HPLC or mass spectrometer.
Amount of Sample 100 pmol
Structural Integrity Determined by MALDI mass spectrometry and HPLC.
Labeling Selectivity Essentially stoichiometric labelling. One fluorophore molecule covalently bonded to each glycan.
Detection Fluorescence detection: 320 nm excitation wavelength, 420 nm emission wavelength.
Purity >88% pure as assessed by a combination of 1 H-NMR and HPLC.
Storage -20˚C both before and after dissolution. This product is stable for at least 5 years as supplied.
Shipping The Alpha Gal 2-AB glycan can be shipped at ambient when dry.
Handling Allow the unopened vial to reach ambient temperature and tap unopened on a solid surface to ensure that most of the lyophilized material is at the bottom of the vial. Gently remove the cap, add the desired volume of reconstitution medium, re-cap and mix thoroughly to bring all the oligosaccharide into solution. For maximal recovery of oligosaccharide, ensure that the cap lining is also rinsed and centrifuge the reconstituted vial briefly before use. Ensure that any glass, plasticware or solvents used are free of glycosidases and environmental carbohydrates. Minimise exposure to elevated temperatures or extremes of pH. High temperatures and low pH will cause desialylation. High pH will cause epimerisation of the reducing terminus GlcNAc.
Safety: This product is non-hazardous and has been purified from natural sources certified to be free of all hazardous material including pathogenic biological agents.