Latest articles

New method for glycan analysis available from Ludger

10/10/09

Ludger have developed a method for analysis of 2AB labelled glycans using the LudgerSep R1 column. This method is particularly well suited for the following applications:

 

 

·         G0, G1, G2 glycan separation

 

·         fucosylated/non-fucosylated glycan separation

 

·         bisecting GlcNAc/non-bisecting GlcNAc glycan separations

 

·         separating Man 5 from neutral complex bi-antennary glycans

 

In addition this method reduces analysis time by a factor of two (compared to the original method). Two columns joined with a zero dead volume connector are required for this application.

 

Please contact us for more details.

New Quantitative Monosaccharide Analysis Kit

14/09/09

Ludger Ltd is delighted to announce the launch of a new system for quantitative monosaccharide analysis of biopharmaceuticals. This is comprised of two parts; a kit for release and fluorescent labelling of monosaccharides and an HPLC column for analysis.


Ludger Ltd is delighted to announce the launch of a new system for quantitative monosaccharide analysis of biopharmaceuticals. This is comprised of two parts; a kit for release and fluorescent labelling of monosaccharides and an HPLC coumn for analysis. The kit (Catalogue No. LT-MONO-96) can be used in either of two modes - full quantitative monosaccharide analysis (e.g. for regulatory submissions) or routine monosaccharide analysis (e.g. for optimisation of glycosylation during clone screening and scale-up). Samples for analysis can be intact therapeutics or oligosaccharides pre-released from the glycoprotein. The kit contains reagents for release and fluorescent labelling of neutral and amino monosaccharides alongside quantitative reference standards. Analysis of the labelled monosaccharide residues is then performed using the LudgerSep R1 column (Cat No. LS-R1-4.6x150) and quantitation is achieved using calibration curves obtained with the standards.

 

For more details e-mail sales@ludger.com .

 

Ludger to launch two Ludger Liberate kits for release of glycans

27/07/09

Ludger will soon be launching two new kits for releasing glycans from glycoproteins. These Ludger Liberate kits have been developed as tools for biopharmaceutical glycoprofiling and can be used for monitoring glycosylation throughout all the stages of the Drug Life Cycle.

Ludger Liberate™ Hydrazinolysis Kit (catalog number LL-HYDRAZ-A2):

 

Hydrazinolysis is a very well established method for the non-selective release and recovery of intact glycans from glycoproteins. The hydrazinolysis method is used in specialist laboratories for glycosylation analysis of approved biopharmaceuticals and follow up biosimilars.

 

  • The Ludger Liberate™ Hydrazinolysis Kit makes this practical method accessible to general R&D analytical labs to enable glycan characterisation during drug development, batch to batch comparability and support for regulatory submissions and for drug patents.
  • Allows release of both N- and O-linked glycans. Reaction conditions can be chosen for the release of either O-glycans, N-glycans or N+O glycans.
  • Standardized and qualified reaction components ensure consistent and reliable assay performance.
  • Released glycans have free reducing termini that are suitable for fluorescent labelling prior to analysis by HPLC, CE and MS. Fluorescent labelling allows relative quantitation of glycan species by HPLC.

Ludger Liberate™ Orela Kit (catalog number LL-ORELA-A2):

 

The Ludger Liberate™ Orela Kit has been developed for the release of O-linked glycans from glycoproteins.

 

  • Designed for easy, routine analysis of O-glycosylation by QC labs.
  • Straightforward and simple method.
  • Released glycans have free reducing terminii suitable for fluorescent labelling prior to analysis by HPLC and MS. Fluorescent labelling allows relative quantitation of glycan species by HPLC.
  • Faster and lower cost than hydrazinolysis.
  • No special handling techniques.

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