Glycan Quantitation

Part of Ludger’s range of System Suitability Standards and Controls, our BioQuant range has been designed for glycan quantitation.

Purity of BioQuant standards is determined as > 90% by UHPLC. Correct mass identity is assessed by MALDI mass spectrometry. The exact amount of material/concentration is determined by quantitative NMR (qNMR) and quantitative monosaccharide analysis (MA). Quantity values by qNMR and MA agree within 90-110%. MA is traceable to internationally accepted references from USP and dispensed using NIST traceable labware. qNMR is traceable to a NIST SRM traceable Certified Reference Material which has been analysed to the ISO 17025 standard.

A detailed Certificate of Analysis is given for each BioQuant standard which contains comprehensive documentation, lot-specific values, expiration date and storage information.

The presentations below relate to BioQuant chitotriose and our sialylated glycopeptide standards and how you can use them:



Ludger BioQuant Chitotriose Ludger BioQuant GPEP-A2G2S2



  Chitotriose Quick Links:

  1. Introduction

  2. BioQuant Chitotriose Structure, Workflow, and Components

  3. BioQuant Chitotriose Data Analysis

  4. Next Steps

  GPEP-A2G2S2 Quick Links:

  1. Introduction

  2. Structure, Quantity, Purity

  3. Monosaccharide Analysis using BioQuant GPEP-A2G2S2

  4. Sialic acid analysis using BioQuant GPEP-A2G2S2

  5. Next Steps
























Ludger's BioQuant Range of Products

Cat #

Quantitative unlabelled chitotriose standard. Use as an internal standard by adding to your glycan samples before labelling:

BQ-CHITOTRIOSE-01     BioQuant Chitotriose standard


Quantitative labelled chitotriose standards. Use as an external standard by running directly on the HPLC/UHPLC:

BQ-CAB-CHI-01     BioQuant 2AB labelled Chitotriose standard
BQ-CAA-CHI-01     BioQuant 2AA labelled Chitotriose standard

Quantitative sialylated glycopeptide standard. Use during the sialic acid or monosaccharide release and labeling process to
check the efficiency of glycan release, labeling and recovery:

BQ-GPEP-A2G2S2-10U     BioQuant GPEP A2G2S2 glycopeptide standard





Contacts

Technical Questions

Simon Peel

Simon Peel
Senior Scientist
simon.peel@ludger.com

Quotations and Orders

Karen Oakes

Karen Oakes
Sales Manager
Quotations: info@ludger.com
Orders: sales@ludger.com